Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Karyotype Analysis and Research of Infection Process of Rhizoctonia solani AG-1 IC

Rongtao Fu, Mingjing Zhou, Chuanchun Yin, Aiping Zheng* and Ping Li

Rice Research Institute, Sichuan Agricultural University, 211 Huiming Road, Wenjiang District, Chengdu City, Sichuan Province, China.

Received on 08 July 2013 and accepted on 16 August 2013

 

ABSTRACT

Rhizoctonia solani AG-1 IC (teleomorph: Thanatephorus cucumeris) is an important soilborne plant pathogenic fungus. This study carried out the morphological feature observation of AG-1 IC, karyotype analyses, and infection process observation of Chinese kale and soybean. We found the sclerotia consisted of dense mycelium using scanning electron microscopy. For the most part, per cell of hyphae contained between 4 and 6 nuclei, and in most cases, each nucleus possessed 12 or 13 chromosomes. Symptom development on Chinese kale and soybean by AG-1 IC was studied. The disease symptoms of Chinese kale were more serious than soybean. AG-1 IC grew over the plant, adhered, and formed infection cushions and appressoria. Multiple invasions from the epidermal cell and hyphal invasion Chinese kale through stoma were observed.

Keywords : Thanatephorus cucumeris, Karyological analysis, Chromosome, Appressorium.