Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Genome Shuffling of Saccharomyces cerevisiae for Improving Thermotolerance and Glycerol Production

Long Miao1,2, He Runxia2, Dong Shuang2, Jing Wenying2 and Huang Kehe1*

1College of Veterinary Medicine, Nanjing Agricultural University,Nanjing, Jiangsu, 210095, China. 2College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang Liaoning, 110161, China.

Received on 14 June 2013 and accepted on 02 August 2013

 

ABSTRACT

In this study, the thermotolerant and high glycerol-producing Saccharomyces cerevisiae strain F3/24 was obtained after three rounds of genome shuffling of UV and NTG mutants derived from the original strain Saccharomyces cerevisiae NAU-ZH-GY1. The recombinant S. cerevisiae strain F3/24 could grow well at 45 ?C. At temperature higher than 39?C, recombinant strain F3/24 always gave higher cell growth and glycerol production. In fermentation medium containing 200g/l glucose and 5g/l corn steep liguor powder, glycerol yield of the shuffled strain F3/24 was 75.3, 72.4, 70.5 and 68.7g/l at 32 ?C, 39 ?C, 42 ?C and 45 ?C for 64h, respectively.

Keywords : Genome shuffling, Saccharomyces cerevisiae, Glycerol, Thermotolerance.