Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Micropropagation of Seven Stevia rebaudiana Bert. Genotypes Via Adult Leaf Explants

Mohamed El-Zaidy1, Mohamed Zayed1, Sulaiman Alharbi1, Abdullah Doaigey1, Abdulaziz Al Sahli1 and Reda Ammar2

1Department of Botany and Microbiology, 2Department of Chemical Sciences, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.

Received on 11 January 2014 and accepted on 09 March 2014

 

ABSTRACT

Stevia rebaudiana Bertoni is a perennial herb belongs to Compositae family used as a natural sweetener for diabetic patients, and it is native to Paraguay. Moreover, the tissue culture techniques were used to identify the best medium for callus induction and the regeneration using different concentrations of phytohormones to produce a high number of shoots and roots in each genotype. Results indicate that the Murashige and Skoog (MS) or (Linsmaier & Skoog) LS medium supplemented with 1.0 mg/l 2,4-D + 0.5 mg/l BAP + 1.0 mg/l GA3 was the favorable for all calli characters and the genotype-4 was the best one for callus induction. The green embryos character was highest in genotypes-4 with the regeneration media containing MS salts + 2.0 mg/l BAP. The high percent of multiplication and elongation shoots were obtained with genotypes-5 and 3 with a medium which containing MS salts + 0.1 mg/l BAP. Finally, the medium which contained MS salts only was the best one for differentiated roots.

Keywords : Stevia rebaudiana, Micropropagation, Callus, Organogenesis, Regeneration.