Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Protective Immune Response in BALB/c Mice Induced by the E72-296 Protein of Rubella Virus

Zhen-Mei Li1, Zi-Hao Sun2, Hong-Ling Wen1, Bin Lin3, Fu-Lu Chu1, Guo-Hong Li4 and Zhi-Yu Wang1*

1Department of Virology, School of Public Health, Key Laboratory for Experimental Teratology of the Ministry of Education, Shandong University School of Medicine. 2The Mental Hospital of Jinan City, Jinan, China. 3Shandong Center for Disease Control and Prevention, Jinan,China. 4Jinan Central Hospital, Jinan, China.

Received on 18 February 2014 and accepted on 21 April 2014

 

ABSTRACT

The goal of this study is to assess the protective efficiency of the E72-296 protein, a candidate for a safer rubella virus vaccine. BALB/c mice were immunized with purified E72-296 protein, RV JR23 strain or Attenuated RV Vaccine. Anti-RV antibodies were tested by indirect ELISA, and cell immune responses were examined by lymphocyte proliferation assay. NK cell activity and challenge tests were assayed to evaluate the safety of the E72-296 protein. In this study, the RV-specific ELISA antibodies induced by the E72-296 protein were obtained. Mice immunized with the E72-296 protein had effective protection against RV when challenged with RV JR23 ten days after triple immunization. Further analyses of cell-mediated immune responses showed that the E72-296 protein induced positive cell-mediated immunity (CMI) (stimulation index ?2.0 ). In addition, it was noticed that E72-296 protein could not alter splenic NK cell cytotoxicity, which was reduced in mice immunized with the Attenuated RV Vaccine. This suggested that E72-296 protein was safer than latter. These results indicated that the E72-296 protein not only induced complete protection against RV challenge in mice, but is also a candidate for a safer vaccine.

Keywords : Rubella virus; Pichia pastoris; E72-296 protein; NK cell activity; Protective immune response.