Journal of Pure and Applied MicrobiologyVol. 6 No. Special Edition Oct. 2012

Assessment of Interleukin-10 in Leptospira Associated Acute Hepatic Syndrome

Meher Rizvi?*#, M. Azam?#, Asfia Sultan?, Fatima Khan?, Indu Shukla?, Abida Malik? and M.R. Ajmal?

?Department of Microbiology, ?Department of Medicine, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.

Received on 04 March 2012 and accepted on 10 June 2012

 

ABSTRACT

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira and is characterized by a broad spectrum of clinical manifestations of varying severity ranging from inapparent infection to fulminant, fatal disease. Although several components of this organism have been identified, the molecular mechanisms underlying pathogenesis of this infectious disease are still poorly understood. Besides, direct injury by microbial factors, cytokines produced in response to infection have been proposed to be involved in pathogenesis of leptospirosis. The present study was done to document the prevalence of leptospira induced acute hepatitis and to estimate the levels of Interleukin-10 in these patients. 247 consecutive cases with symptoms of acute hepatitis and 30 healthy controls were enrolled in the study and detailed clinical history was elicited from them. ELISA for HAV, HBV, HCV and HEV were performed to rule out common viral etiology of hepatitis. IgM antibodies to leptospira were detected by ELISA. IL-10 levels were estimated in leptospira positive cases by ELISA. Out of 247 cases of acute hepatitis 46 (18.62%) were observed to be positive for IgM antibodies for leptospira. ALT, AST, ASP was raised in majority of patients. IL-10 was found to be elevated in a large majority of cases 56.52% (26/46). Patients with more severe symptoms were associated with higher levels of IL-10.

Keywords : Leptospirosis, IL-10, Hepatitis, ELISA.