Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Production of poly-(3-hydroxybutyrate) by Native Bacillus Isolates from Agricultural Wastes

M. Thirumala*, S. Vishnuvardhan Reddy and S.K. Mahmood

Environmental Microbiology Lab, Department of Botany, Osmania University, Hyderabad - 500 007, India.

Received on 26 February 2009 and accepted on 20 April 2009



Two native Bacillus isolates produced PHB using agricultural wastes like wheat straw, rice straw, sugarcane bassage, corn cobs and saw dust. Bacillus sp. 87I produced 50.9% (1.5 g/l PHB), when sugars from sugarcane bassage were used in E2 media. The second Bacillus sp. 112A produced 47.5 % (1.1 g/l) of PHB, when filtrate from sugarcane bassage fermentation was used as carbon substrate. The native Bacillus spp. 112A and 87I have desirable properties of tolerance to high conditions of pH and temperatures.

Keywords : Agricultural wastes, Bacillus sp., Isolates, PHB, Production.