Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Two Step Production of Optimized Interferon Beta 1b; A Way to Overcome its Toxicity

Mohammad Ali Mobasher1,2, Younes Ghasemi1,2*, Nima Montazeri-Najafabady1,2, Abdollah Ghasemian1,2, Sara Rasoul-Amini1,2,3, Shiva Hemmati1,2 and Sirus Ebrahimi4

1Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345 1583, Shiraz, Fars, Iran. 2Department of Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345 1583, Shiraz, Fars, Iran. 3Department of Medicinal Chemistry, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345 1583, Shiraz, Fars, Iran. 4Department of Chemical Engineering, Sahand University of Technology, P.O. Box 53317 11111, Tabriz, Iran.

Received on 21 January 2013 and accepted on 25 February 2013

 

ABSTRACT

Interferons are a large group of biological substances with many important functions like immunomodulatory, antiviral and anti inflammatory activities. There are so many clinical applications for IFN b for example in treatment of multiple sclerosis (MS), hepatitis, genital condylomata acuminate and arthritis. In this study the optimized IFN b 1b for expression in E.coli BL21 was designed and coloned in pET15b. In general, because of its toxicity and instability the yield of expressed IFN b 1b is low, so the amounts of expression and the effect of co products on production of IFN b 1b were evaluated and a two step production condition was designed for more efficacy in expression of this important biological recombinant product.

Keywords : Interferon, Optimization, cloning, Multiple sclerosis, E. coli.