Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Proteomic and Genetic Diversity of Mycoplasma hyopneumoniae Isolates from South Korea

Abhijit K. Barate1, Gyu-Seong Jang1, Seongbeom Cho2 and Tae-Wook Hahn1*

1Department of Veterinary Medicine, College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, 200-701, Republic of Korea, 2 Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

Received on 04 March 2013 and accepted on 15 April 2013

 

ABSTRACT

The purpose of the present study was to evaluate the diversity of Mycoplasma hyopneumoniae (Mhp) isolates from South Korea. Overall, 42 field isolates recovered from 36 herds were analyzed using sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and random amplification of polymorphic DNA (RAPD). Fifteen highly virulent strains were identified based on the presence of a 181 kDa band, a protein band associated with high virulence. A variability of 25.3% and 35.5% was noticed between field strains and J strain at protein and genomic level, respectively. Among the field strains, a variability of 22.2% and 31% was observed at protein and genomic level, respectively. Furthermore, isolates from the same herd demonstrated differences in protein and RAPD patterns. These results reveal high proteomic and genetic diversity among the field isolates of South Korea.

Keywords : Enzootic swine pneumonia, Mycoplasma, Typing, SDS-PAGE, RAPD.