Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Catalytic Zone, A Novel Screening Approach for the Production of Extracellular Tyrosinase by Streptomyces vinaceusdrappus DSV5

S. Shivaveerakumar1,2, D.N. Madhusudhan1, Agsar Dayanand1* and M.H. Kontro2

1Department of Microbiology, Gulbarga University, Gulbarga, 585 106, Karnataka, India. 2Universtiy of Helsinki, Department of Environmental Sciences, Niemenkatu 73, 15140 Lahti, Finland.

Received on 30 January 2013 and accepted on 03 March 2013

 

ABSTRACT

An investigation was carried out to search potential actinobacteria for tyrosinase activity from different environmental samples. Detection of a typical catalytic zone developed on Tyrosine Gelatin Beef extract (TGB) agar indicating the tyrosinase activity by actinobacteria is a unique approach, which was never reported earlier. A total of five isolates of actinobacteria obtained from soils (DSV15 and DPG2) of Gulbarga India and marine sediments (DSV5) from Goa India were proved to be promising during primary and secondary screening for tyrosinase activity, based on the degree of catalytic zone and also intensity of color. Thus, observation of catalytic zone in addition to the intensity of color is a novel approach for the detection of tyrosinase activity. TGB medium has been proved as most suitable (high intensity of color and maximum catalytic zone) for tyrosinase activity, from among eight media, including two conventional and seven modified media, assessed during primary and secondary screening. An isolate of actinobacterium DSV5 has been proved as most efficient with the maximum production of tyrosinase in both Starch Tyrosine mineral (160?0.2U/ml) and TGB (175?0.2U/ml) media. The most potential isolate DSV5 was confirmed as Streptomyces vinaceusdrappus based on the molecular characterization by 16S rRNA analysis.

Keywords : Streptomyces, Tyrosinase, Screening, Pigmentation, Catalytic zone, Production.