Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Solid State Protease Production by Bacillus thuringiensis AP - CMST using Trash Fish Meal as Substrate

P. Esakkiraj, T. Maruthiah, P. Mohideen Askar Nawas1, G. Immanuel and A. Palavesam*

Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamilnadu, India. 1V.S.Sivalingam Government Arts College, Pulankurichi, Sivagangai - 630 413, India.

Received on 07 February 2013 and accepted on 25 March 2013

 

ABSTRACT

A proteolytic Bacillus thuringiensis AP-CMST was isolated from the gut of estuarine fish Etroplus suratensis and the factors affecting protease production in solid-state fermentation was assessed using trash fish - Odonus niger meal and the production was utmost in pH 7 and temperature 40?C. The effect of carbon and nitrogen sources on the protease production indicated that it was maximum in maltose and beef extract supplied medium. The other suitable nutrients observed for better protease production were Tween 60 and magnesium sulphate. The inorganic phosphates have little influence on protease production, though it was maximum in diammonium hydrogen orthophosphate added medium. The effect of various concentrations of NaCl indicated that the maximum enzyme production was noticed in 4% of NaCl.

Keywords : Protease, Solid-State Fermentation, Trash Fish Meal, Beef Extract.