Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Study on the Antimicrobial Effects of Aqueous Extracts from Portulaca oleracea L

Shuxia Ma1, Junxing Liu1, Lan Feng2, Chunmin Wang1, Jiqiu Ren1, Chunjia Yang 1, Yue Dai1, Gang Cui1, Kun Zhang1, Chengwei Li1, Xiang Cheng1, Lianshun Cai1, Fei Wu1, Lin Wang3 and Zhiwei Yang1*

1School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, P. R. China. 2Clinical Medical College, Jiamusi University, Jiamusi 154007, P. R. China. 3The First Affiliated Hospital of Jiamusi University, Jiamusi 154003, P. R. China.

Received on 21 March 2013 and accepted on 19 May 2013

 

ABSTRACT

Portulaca oleracea L. is distributed across China and used a long time ago in clinic for the treatment of microbial infections, as a traditional Chinese herbal medicine. This work was performed in order to evaluate the antimicrobial activities of aqueous extracts from Portulaca oleracea L.. The MIC and MBC determinations combined with confocal laser scanning analysis were used to study the antimicrobial activities of aqueous extracts, against the common pathogens of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. The microorganisms were incubated on the biofilm-grown and planktonic stages, respectively.It was found that the extracts had the great potential inhibiting activities against all 3 microorganisms, with the MIC value of 0.25 %. As to these microorganisms, Staphylococcus aureus has the lowest MBC value (0.125 %). Further, the CLSM data reveal that the antimicrobial effects of the extracts are with the concentration- and time-dependent relationships, and there is no linear relationship between bacterial biofilm inhibition activities and concentration of the extracts. Therefore, the aqueous extracts from Portulaca oleracea L. might be considered as a potential antimicrobial agent.

Keywords : Portulaca oleracea L, Aqueous extracts, Antimicrobial, MIC, MBC, Confocal laser scanning.