Journal of Pure and Applied MicrobiologyVol. 7 No. 4

In vitro Susceptibility Test of Antifungal Drugs for Isolated Aspergillus Species from Aspergillosis Patients in Riyadh City, Saudi Arabia

Khalid Niazi1, Saleh A. Kandeal2,4, Abdulla S. Khalel1 and Jamal M. Khaled1,3*

1Department of Botany and Microbiology, 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. 3Department of Biotechnology and Food Technology, Thamar University, Yemen. 4Anim. Prod. Dept. Faculty of Agriculture, Cairo University.

Received on 30 August 2013 and accepted on 02 October 2013

 

ABSTRACT

The purpose of this study was to isolate and identify the most important species belonging to Aspergillus genus that cause aspergillosis disease in Riyadh city, Saudi Arabia and determine the best specimens taken from patients to isolate the Aspergillus, as well as to specify the best antifungal drugs for its inhibition, also to identify which of these isolate species are more resistant to these antifungal agents. A total of 53 samples was collected from patients initially diagnosed as infected with Aspergillosis disease. These samples were from sputum, tracheal aspirate, nasopharyngeal aspirate, bronchoaveolar lavage and lung tissue. Direct examination, culturing process, isolation of the fungus were performed to identify the isolates by their micro- and marocharacteristics. To confirm the identification, the isolates were sent to Assiut University Mycological Center (AUMC) in Egypt. In vitro susceptibility test of different antifungal (Amphotericin B, Anidulafungin, Caspofungin, Fluconazole, 5-Flucyosine, Itraconazole, Micafungin, Posaconazole and Voriconazole) for isolated Aspergillus was carried out. The results revealed that the most fungal isolates were obtained from sputum samples followed by aspirate samples from trachea. The cause of aspergillosis disease was confined to the five following species: A. fumigatus, A. flavus, A nidulans, A. niger and A. terreus. The most frequent isolate was A. fumigatus. The antifungal caspofungin and anidulafungin were the most effective ones, whereas fluconazole and 5-flucyostine were the least effective antifungal drugs. It was also found that A. fumigatus was the most resistant species among all studied isolates.

Keywords : Aspergillus, Aspergillosis, Fumigatus, Caspofungin, Anidulafungin.