Journal of Pure and Applied MicrobiologyVol. 7 No. 4

16S rRNA Molecular Identification of Metal Tolerant Bacteria Isolated from Electronic Waste Recycling Facility and its Heavy Metal Removal Ability

Ramasamy Rajesh Kumar1,3+, Bung Ju Park2+ and Jae Young Cho3*

1Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappali 620 024, India. 2Department of Horticultural Science, Chungbuk National University, Cheongju, Chungcheongbuk-do, 361 763, Republic of Korea. 3Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Jeollabuk-do, 560 756, Republic of Korea.

Received on 23 January 2013 and accepted on 04 March 2013

 

ABSTRACT

The lead nitrate removal by isolated lead tolerant bacteria Pseudomonas sp isolated from electronic waste (e-waste) recycling facility soil was studied in a laboratory simulated environment. The bacterium was identified by biochemical and 16S rRNA molecular studies. The DNA was isolated and amplified using universal bacterial primers. The phylogeny tree suggested the isolated lead tolerant bacterium as Pseudomonas aeruginosa. The partial 16S rRNA sequences were submitted to NCBI Genbank and the accession number JN102340 was assigned to the submitted sequences. Various parameters were optimized for lead nitrate removal such as pH, temperature, kinetics studies and metal tolerant assay. The metal removal studies suggested that the lead tolerant Pseudomonas aeruginosa JN102340 able to remove lead nitrate 92% at pH 6, 89% at 35?C, 90% after 24h incubation in the optimized pH and temperature. The bacterium Pseudomonas aeruginosa JN102340 tolerate and survive up to 600mg L-1 lead nitrate.

Keywords : Bioremediation; Lead removal; Pseudomonas aeruginosa; Bacteria; e-waste; 16S rRNA.