Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Ethanol Production from Rice (Oryza sativa) Straw Biomass by Separate Hydrolysis and Fermentation

Annu Goel and Leela Wati*

Department of Microbiology, CCS Haryana Agricultural University, Hisar-125 004, India.

Received on 18 February and accepted on 21 April 2013

 

ABSTRACT

This paper presents a study on the use of rice (Oryza sativa) straw biomass for ethanol production. Alkali treated rice straw was successfully converted to ethanol using commercial cellulase and Saccharomyces cerevisiae HAU-1 strain. Treatment of 0.5 mm straw with 2% sodium hydroxide at 15 psi for 1 h resulted in 70% lignin removal along with 88% cellulose recovery. The optimum concentration of cellulase enzyme and hydrolysis temperature was found to be 7.5 FPU/ g substrate at 50?C that resulted in about 75% sugar release from polysaccharides. Fermentation of enzymatic hydrolysate by Saccharomyces cerevisiae (HAU-1) resulted in production of 20.46 g/l ethanol after 72 h incubation at 30?C.

Keywords : Bioethanol, Rice straw, Saccharomyces cerevisiae, Fermentation.