Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Isolation and Identification of Methicillin-resistance Staphylococcus aureus (MRSA) from Nasal of College Students

Razieh Amini1,2, Abdulamir AS3, Nurul Azreen B. Mustaffa4, Fatemeh Jahanshiri4, Beh Poay Ling4,5, Ali Hematian6, Morvat Taheri-Kalani6, Yasaman Amini7, Iraj Pakzad6, Zamberi Sekawi5 and Farid Azizi Jalilian6*

1Department of Microbiology, Faculty of Science, Islamic Azad University, Hamedan Branch, Hamedan, Iran. 2Clinical Microbiology Research Center, Ilam University of Medical Sciences, 69391 IUMS, Ilam, Iran. 3Microbiology Department, College of Medicine 70030, Alnahrain University, Baghdad, Iraq. 4Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 5Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 6Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, 69391 IUMS, Ilam, Iran. 7Department of Infectious Disease, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Received on 21 November 2012 and accepted on 26 December 2012

 

ABSTRACT

In this study, classical and molecular methods were involved for isolation and identification of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA). Identification of S.aureus involved tests such as Mannitol fermentation test, coagulase test and DNase test. Antibiotic susceptibility test which was carried out to explore MRSA antibiotic susceptibility patterns. Antibiotics involved were methicillin, oxacillin, vancomycin, tetracycline, cefoxitin, erythromycin, gentamycin, chloramphenicol, penicillin, ampicillin, and trimethoprim. Polymerase chain reaction (PCR) was performed to obtain more reliable result. Among 100 samples collected, totally 18 samples were confirmed as S. aureus based on molecular method. From these isolates, 16 of them showed presence of mecA gene and also fem gene(gene involved in the resistant of methicillin) thus making it positive as MRSA.

Keywords : MRSA, nasal, Staphylococcus, antibiotics.