Journal of Pure and Applied MicrobiologyVol. 7 No. 2

Enzyme-linked Immunosorbent Assay for Detection Staphylococcal Enterotoxins in Synovial fluid of Rheumatoid Arthritis Pateints

Ramezan Ali Ataee1, Zyenab Mohoseni-Moghadam3, Ahmad Salimzadeh4, Ali Mohammad Latifi5, Mohamad Hossein Ataee1 and Gholam Hossein Alishiri2*

1Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. 2Department of Rheumatology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IR Iran. 3Department of Toxicology, Azad University, Tehran, IR Iran. 4Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. 5Applied Biothechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.

Received on 30 July 2012 and accepted on 10 September 2012

 

ABSTRACT

Experimental evidence suggests that staphylococcal superantigenic enterotoxins are involved in arthritis. The aim of this study was to detect staphylococcal enterotoxins in synovial fluid of seronegative rheumatoid arthritis patients. A total fifty synovial fluid samples from patients with seronegative rheumatoid arthritis were analysed. A commercial sandwich-enzyme immunoassay for the detection of Staphylococcus aureus enterotoxins SET A, B, C, D and E were used. The samples were separately processed by Amicon Ultrafiltration system. The results were confirmed by western blotting test. The results indicated that, more than 60 percent of the synovial fluid smaples of the patients with rheumatoid arthritis have at least one of the staphylococcal enterotoxins. Based on our study, the most abundant enterotoxin seen in the synovial fluid of patients with rheumatoid arthritis were enterotoxin A, C, E, B and D respectively in order of presence. However, bacteriological culture was negative for Staphylococcus aureus isolation. The results showed the existence of Staphylococcal enterotoxins in the synovial fluid of seronegative rheumatoid arthritis patients. While none of the samples, bacterial growth was observed. This finding could provide a new test method to diagnosis of rheumatoid arthritis and was designed based on the specific treatment of disease. In addition, these findings suggest that, detection of Staphylococcal enterotoxins in synovial fluid as a new biomarker can help identify causes and select specific traetment options.

Keywords : Elisa, Staphylococcus aureus, Enterotoxins, Western-blotting, Rheumatoid Arthritis, Seronegative.