Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Cloning and Expression of Dissimilatory Sulfite Reductase (dsrAB) of Desulfotomaculum geothermicum Isolated from Indian Petroleum Refinery Cooling Towers

B. Anandkumar¹*, E.S. Challaraj Emmanuel² and S. Maruthamuthu³

¹Department of Biotechnology, ²Department of Microbiology, Sourashtra College, Madurai - 625 004, India ³Corrosion Protection Division, Central Electro-Chemical Research Institute, Karaikudi - 630 001, India.

Received on 13 July 2008 and accepted on 18 September 2008

 

ABSTRACT

A thermophilic sulfate reducing bacterium was isolated from corrosion product of cooling tower of an Indian petroleum refinery and characterized as Desulfotomaculum geothermicum with morphological and biochemical characters. Gene coding dissimilatory sulfite reductase (dsrAB), a key enzyme in the sulfate reduction was sequenced and cloned in an expression vector and over expressed with rDNA technology. Purified protein was identified as dissimilatory sulfite reductase having the molecular size of 97.6 kDa with MALDI - MS and discussed.

Keywords : Sulfate reducing bacteria, biocorrosion, dissimilatory sulfite reductase.