Journal of Pure and Applied MicrobiologyVol. 7 No. 1

Study on Endotoxin Removal from Human-like Collagen

Huizhi Zhang1, Daidi Fan1*, Jianjun Deng1, Xiaoxuan Ma1, Chenghui Zhu1 and Wenjiao Xue2

1Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi?an, Shaanxi, China. 2Shaanxi?Provincial?Institute?of?Microbiology,?No.?76?Xiying?Road,?Xi?an?- 710 043, China.

Received on 11 January 2013 and accepted on 27 February 2013

 

ABSTRACT

In this study endotoxin is removed from protein preparations using a Pierce? High-Capacity Endotoxin Removal Resin column. The effects of phosphate buffer and NaCl concentration on endotoxin removal and protein recovery have been investigated. Both PBS concentration and NaCl had significant effects on endotoxin removal from protein solutions. When PBS and NaCl were increased from 10 to 40mM and from 100 to 200mM respectively, the residual endotoxin was reduced from 8000 to 25 EU/ml in the PBS 25mM and NaCl 150mM. When protein concentration was varied from 1 to 5mg/ml, the residual endotoxin was reduced to about 20EU/ml in the PBS 25mM and NaCl 150mM. However, the incubation time had a critical effect on the endotoxin removal. Protein recovery was above 98% in all cases. This method greatly provided high endotoxin removal and HLC recovery.

Keywords : Endotoxin removal, Human-like Collagen, Endotoxin-protein Complex, Lipopolysaccharides.