Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Antibacterial Activity of Gongronema latifolium and Ocimum gratissimum Extracts on E. coli and Salmonella typhi

U.N. Ekwenye and S.N. Onwukwe

Department of Microbiology Michael Okpara University of Agriculture, P. M. B. 7267, Umuahia, Abia State, Nigeria.

Received on 10 March 2008 and accepted on 05 May 2008

 

ABSTRACT

The antibacterial activity of ethanolic and aqueous extracts of Gongronema latifolium and Ocimum gratissimum on E. coli and Salmonella typhi were determined using disc diffusion method. The antibacterial effect of aqueous extracts of Gongronema latifolium was negative on both test organisms and the aqueous extracts of Ocimum gratissimum showed very little zone of inhibition on E. coli (3.00mm) diameter and on Salmonella (2.00mm) diameter. Ethanolic extracts of both Gongronema latifolium and Ocimum gratissimum showed little zone of inhibition on E. coli and Salmonella typhi, which was not greater than 3.00 mm diameter. The minimum inhibitory concentration (MIC) evaluated with both ethanolic and aqueous extracts of Ocimum and Gongronema of various concentrations observed did not show any zone of inhibition. The results suggested that the extracts have low potential for use in the treatment of enteric diseases caused by pathogenic bacteria.

Keywords : Gongronema latifolium, Ocimum gratissimum, antibacterial activity,