Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Screening of Streptomycetes for Production of Desferrioxamines

Fumiya Kobayakawa1 and Shinya Kodani1,2*

1Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. 2Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.

Received on 10 December 2011 and accepted on 12 January 2012

 

ABSTRACT

Desferrioxamine B is a hydroxamate-type siderophore originally isolated from S. pilosus, and has been used to treat thalassemia major patients by chelating overloaded iron. Multiple reports of production of desferrioxamines in streptomycetes were reported, however the distribution of desferrioxamines were still not clear. To examine the distribution of desferrioxamines in streptomycetes, we performed HPLC screening using 41 streptomyces strains. The production of desferrioxamines was detected in high percentage (78%, 32/41) of streptomycetes, which indicated that desferrioxamines were widely distributed in streptomycetes.

Keywords : Siderophore, Streptomyces, Desferrioxamines, HPLC, ESI-MS.