Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Growth and Lignocellulolytic Enzyme Profile of Different Strains of Lentinus edode with Different Carbon Sources

Mohan Jairath, P.K. Khanna*, Neeta Raj Sharma and Giridhar Soni

Department of Biotechnology, Lovely School of Biosciences, Lovely Professional University, Phagwara, Punjab, India. *Department of Microbiology Punjab Agricultural University, Ludhiana, Punjab, India.

Received on 20 March 2012 and accepted on 10 May 2012

 

ABSTRACT

Lentinus edodes (Berk.), the Shiitake mushroom, is highly prized for nutritive and medicinal values. It is traditionally cultivated on the wood logs of Shii tree in Japan. Non availability of the Shii tree in India has necessitated a search for alternative substrates for shiitake cultivation. The investigation focuses on the possibility of using some of the locally available lignocellulosics materials as carbon source in growing media. Three strains of shiitake mushroom (LeC, LeS, OE329) were cultivated on rice husk, wheat bran, corn-cobs and sugarcane bagasse as carbon sources. The maximum linear mycelial growth was observed in LeC strain followed by LeS while a slower mycelial extension was observed in OE329 strain. Among all the three strains endoglucanase activity was found to be highest for LeC (corn-cobs) followed by LeS (corn-cobs) and OE329 (rice husk), while cellobiase activity was maximum with rice husk followed by corn cobs among all the three strains (LeC, LeS, OE329) and maximum exoglucanases was released by LeC followed by LeS and OE329 on their culturing on rice husk as carbon source. Wheat bran as carbon sources resulted in maximum laccases activity and that was on par with rice husk. Thus all the three strains of L.edodes showed the preference for rice husk as carbon source. LeC is the only strain that showed maximum growth and enzyme activity on all the C sources tried. There appears to be a direct correlation between the growth and enzyme released by different strains on different carbon sources.

Keywords : Cellulases, Laccases, Lentinus edodes, Mycelial growth, Shiitake.