Journal of Pure and Applied MicrobiologyVol. 8 No. 4

Influence of Culture Conditions on Production of Natural Antibacterial Red Pigment Produced by Serratia marcescens IBRL USM 84

I. Darah1, K. Jain2 and Lim, Sheh-Hong1*

1School of Biological Sciences, 2School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.

Received on 18 February 2014 and accepted on 21 April 2014

 

ABSTRACT

This study was undertaken to investigate the influence of cultural conditions on the production of antibacterial red pigment by Serratia marcescens IBRL USM 84. This isolate exhibited maximal antibacterial red pigment production of 37.17 U/ml which was achieved at 40 hours of cultivation time when cultivated in marine broth with improved conditions, including the addition of 0.2% (w/v) of agar, cultivation temperature at 25?C, initial medium pH of 7, agitated at 150 rpm and 2% inoculum size (1x109 cells/ml). There was an increment of 164% of red pigment production after improvement compared to before improvement of cultural conditions. The results suggested different cultural conditions have a significant impact on the secondary metabolite production by the bacterium to gain a high yield.

Keywords : Serratia marcescens; antibiotic; red pigment; marine microorganism.