Journal of Pure and Applied MicrobiologyVol. 11 No. 4

FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition

S. Umamaheswari, S. Malkar Oli and K. Naveena

P.G and Research Department of Zoology, Periyar EVR College, Tiruchirappalli - 620 023, Tamil Nadu, India.

Received on 10 November 2017 and accepted on 21 December 2017

 

ABSTRACT

The demand for cellulose is accelerating in the paper making industry. Alternate sources of cellulose has to be traced in order to reduce the demand for plant cellulose. Hence, in this study bacterial cellulose has been chosen as an option. In this study, the potential of soil bacteria Lactococcus lactis to produce cellulose has been assessed. The results obtained indicate that the inocula size of the bacteria had a vital role in altering the quantity of cellulose produced. Among the inocula size, 100 Ál of broth culture exhibited highest production of cellulose. The cellulose produced was characterised spectrally and its microarchitectural study reveal its crystalline nature. FTIR spectra of the bacterial cellulose produced depict the signature peak of bacterial cellulose. Based on these observations, it could be concluded that Lactococcus lactis produce cellulose. Further studies has to be carried out to optimize the bacterial cellulose production.

Keywords : Lactococcus lactis , cellulose, glucose, FTIR, SEM.