Different solvents were used for sumac extractions to find different active compounds in each fraction, and these fractions were tested on Staphylococcus aureus (which isolated from soft white cheese), the (MIC) minimum inhibitory concentration of each fraction were measured then sub-inhibitory concentration (SIC) was used. We found that all fractions can inhibit quorum sensing in Staphylococcus aureus in a different ratio. By using Real-time PCR found that the different sumac fractions can inhibit the expression of tested genes (Sea, Seb, AgrA, RNAIII, and Hla). Furthermore, most sumac extracted fractions have the ability to decreasing biofilm and growth curve in Staphylococcus aureus significantly, while other fractions decreased them non-significantly.