The present study was included isolating alkaloid from the fruit of the nutmeg (Myristica fragrans) where observed the presence one of alkaloid compound in the fruit of the nutmeg , which is the nature of the aromatic and has carbonyl and phenol groups in addition  to the presence of nitrogen . The isolated alkaloid was showed antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram- negative bacteria (Escherichia coli) while the alkaloid compound was showed antifungal effect against Candida albicans and Cryptococcous neoformans. The isolated alkaloid compound was not showed any cytotoxicity of human blood solution in concentration of (10-200 ppm). From this result we concluded that the alkaloid isolated from Myristica fragrans can be used in treatment of various disorders because of the effectiveness of the compound against bacteria and fungi.