Tarun Kumar, Suresh Chandra, Ankit Singh and Yogendra Singh

 

Department of Agricultural Engineering and Food Technology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Modipuram, Meerut – 250110 (U.P.), India.

ABSTRACT

The study was conducted to see the effect of packaging material and storage condition on the physical properties of tomatoes (Lycopersicon esculentum Mill) varieties (Himshikhar and NS – 524). These varietal tomatoes were packaged in High Density Poly Ethylene (HDPE) and stored under refrigerator, BOD incubator and ambient temperatures. Tomato variety Himshikhar packed in HDPE showed minimum shrinkage at ambient temperature followed by refrigerator and BOD condition.

Keywords: Physical properties, TSS, HDPE, refrigerator, shape factor.

INTRODUCTION

Tomato (Lycopersicon esculentum Mill) is the world’s most commercially produced vegetable (Gaware et al., 2010). The first known record of tomato is in the year 1554 in South America, after 200 years it gradually spread to other parts of the world (Kumar et al., 2012). Tomato is rich source of vitamins A, C, potassium, minerals and fibers. Lycopene is a phytochemical nutrient element found in many fruits and vegetables, but excessively found in tomato that imparts natural red colour (Holden et al., 1999). Use of tomatoes is increasing day by day and a variety of products like puree, syrup, paste, ketchup, juice etc. are made. To design and optimization a machine for handling, cleaning, conveying, separation and storing, the physical attributes and their relationships must be known (Mirzaee et al., 2008). Designing such equipment without consideration of these properties may yield poor results.

Therefore the determination and consideration of these properties have an important role (Taheri-Garavand et al., 2009). Among these physical properties, length, width, thickness, mass, volume, projected areas and center of gravity are the most important factors in sizing systems (Mohsenin, 1986). Viswanathan et al., (1997) concluded that properties viz., size, density, moisture and force varied with the variety of the tomato fruits. The per cent seed, pulp and skin content in the fruit also varied with the variety. Varshney et al., (2007) studies the physical and mechanical properties of tomato and revealed that moisture content and weight density of fruits decreased while loss and volume shrinkage increased with storage period. Kaymak et al., (2010) determined the color and several physical characteristics of two common tomato cultivars (Alida F1 and H2274) grown in Erzincan region in Turkey. Taheri-Garavand et al., (2011) studied on some morphological and physical characteristics of tomato used in mass models to characterize best post harvesting options. Li et al., (2011) studied the structural and geometrical properties; Atallah, (2012) conducted study on three different varieties of tomato, Onifade et al., (2013) investigate some physical properties of local variety of tomatoes that are relevant in the handling and processing of the fruits.

HDPE (High Density Poly Ethylene) used as storage materials, since packaging of fruits in polyethylene films results in modified atmosphere which reduced the fruit decay, softening and loss soluble solids during storage. Keeping of fruits in the polyethylene package help in extension of storage life and retention of quality (Salunkhe and Wu, 1973). According to Vidigal et al., (1979) packing in polyethylene bags decreased weight loss and controlled atmosphere storage improves keeping quality in fruits. Kumar et al., (1999) and Sammi and Masud, (2007) also used polyethylene packaging in their investigation to improve the shelf life of tomatoes. To our knowledge, detailed investigations concerning physical properties of tomato in relation with storage conditions and storage material have not been published. Therefore, the aim of this research was to see the effect on physical attributes of tomato due to HDPE as storage material and three different storage conditions. This information provides useful insights into design of processing, packing equipments and transportations for tomato.

Materials   and  Methods

The experiment was conducted at Food Analysis Laboratory of Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (India). Fresh, ripe, red in colour tomatoes, free from disease and insects were procured directly from the farmers of village Dhanju and Lawad. Two varieties of tomatoes namely Himshikhar and NS-524 were used for the present investigation.

Measurement of dimensions: Three linear dimensions namely polar diameter (D1), major diameter (D2) and minor diameter (D3) for all tomatoes were measured using a Vernier Caliper (least count 0.01mm). Polar diameter is defined as the distance between tomato apex and the stem end. Major and minor diameters of the tomatoes are defined as maximum and minimum width respectively in a plane perpendicular to a polar axis (Mohsenin, 1986).

Mass, volume and density: Mass of fresh tomatoes was determined using high accuracy electronic balance. As the tomatoes were numbered the weight of individual tomatoes were recorded every day. The volume of tomato was determined individually by water displacement method using a cylinder of 1 liter capacity. The mass and volume were expressed in ‘g’ and ‘ml’ respectively (1 ml=1cm3). Densities for tomatoes were calculated using the following equation:

—–(1)

Geometrical and morphological properties: Arithmetic mean diameters (AMD), geometric mean diameter (GMD), surface area and sphericity for tomatoes were calculated by using the following equations as suggested by Mohsenin (1986):

—–(2)

—–(3)

—–(4)

—–(5)

(For sphericity D1 = largest diameter)

Shape factor (: Shape factor based on volume & surface area of tomatoes was determined (McCabe and Smith, 1984) as;

     —–(6)

Where,

Where, V= volume                  S= surface area

TSS: Total soluble solids of tomatoes were measured using a hand hold refractrometer.

Packaging and storage

High density poly ethylene (HDPE) as packaging material was used and then samples were stored under three different storage condition viz. ambient temperature, BOD incubator and refrigerator condition.

 

Statistical analysis

The data obtained from various experiments were recorded during the course of study and subjected to statistical analysis as per the method of “Analysis of variance”. The significance and non significance of data obtained from various experiments was judge with the help of F (Variance ratio) table. OPSTAT software and spreadsheet software (Microsoft Office excel-2007) were used to analyze the recorded data.

Results   and  Discussion

Tomato of variety Himshikhar stored under ambient and BOD incubator condition in HDPE shows a gradual decrement in mean values of entire physical parameters viz. polar diameter (4.80 – 3.63 cm), major diameter (5.80 – 4.28 cm), minor diameter (5.35 – 3.90 cm), AMD (5.317 – 3.933 cm), GMD (5.299 – 3.922 cm), mass (87.218 – 66.640 g), volume (90.00 – 64.50 ml), sphericity (91.438 – 68.873%), surface area (88.181 – 64.422 cm2), density (0.973 – 0.773 g/cc) and shape factor (1.011 – 0.746), which increase with increase in storage period. Similar trends were reported by Varshney et al. (2007). Some samples were spoiled after day five of storage. Tomato (Himshikhar) stored under refrigerator condition shows decrement in mean values of polar diameter (4.28 – 4.18 cm), major diameter (4.98 – 4.78 cm), minor diameter (4.80 – 4.60 cm), AMD (4.68 – 4.52 cm), GMD (4.673 – 4.508 cm), mass (59.219 – 59.065 g), volume (61.25 – 54.50 ml), shape factor (1.027 – 1.019) and surface area (69.171 – 64.557 cm2) with increase in storage period. Whereas the values of sphericity (94.006 – 94.510 %) and density (0.971 – 1.093 g/cc) shows increment. Result data explicit that the TSS increased (6.200 – 6.575 0brix) with increase in storage time for all the storage conditions. Tomato variety NS-524 stored under ambient temperature in HDPE shows decrement in mean values of physical parameters like major diameter (4.47 – 4.38 cm), minor diameter (4.27 – 4.25 cm), AMD (4.322 – 4.289 cm), GMD (4.320 – 4.288 cm), mass (50.478 – 50.221 g), volume (52.33 – 48.67 ml), surface area (58.842 – 57.975 cm2). Although the samples were spoiled after four days of storage. Whereas the values of shape factor (0.989 – 0.992), density (0.966 – 1.033 g/cc), sphericity (96.738 – 97.859 %) and TSS (4.467 – 5.067 0brix) increased continuously and polar diameter (D1) remain unchanged (4.23 cm) before samples get spoiled. The decrement was observed in entire physical parameters viz. polar diameter (4.33 – 2.77 cm), major diameter (4.50 – 2.93cm), minor diameter (4.37 – 2.73 cm), AMD (4.400 – 2.811 cm), GMD (4.399 – 2.809 cm), mass (50.662 – 32.381 g), volume (51.67 – 30.00 ml), surface area (60.921 – 37.345 cm2), density (0.976 – 0.717 g/cc) and shape factor (0.992 – 0.648) under refrigerator storage conditions. Half the samples were spoiled after five days of storage. Only TSS increased (5.733 – 6.467 0brix) continuously. Data explicit that the tomato (NS-524) stored under BOD incubator condition showed decrement in mean values of the entire physical parameter. The samples were spoiled after 3 days of storage under BOD incubator condition. Density (1.018 – 1.118 g/cc) and TSS (4.700 – 5.033 0brix) increased with increase in storage time but major diameter (D2) shows no change (4.50 cm) during storage.

Table 1: Effect of packaging material (HDPE) and storage condition (ambient temperature) on the physical properties of tomato (variety: Himshikhar).

Tomato: Himshikhar Storage material & condition: HDPE, ambient temperature
Days D1 (cm) D2 (cm) D3 (cm) AMD

(cm)

GMD

(cm)

Sphericity (%) Mass

 (g)

Volume (ml) Surface area

(cm2)

Density

(g/cc)

Shape factor TSS

(0Brix)

1 4.80 5.80 5.35 5.317 5.299 91.438 87.218 90.00 88.181 0.973 1.011 6.550
±0.23 ±0.24 ±0.10 ±0.10 ±0.10 ±2.91 ±10.71 ±14.14 ±3.46 ±0.04 ±0.03 ±0.58
2 4.80 5.65 5.20 5.217 5.203 92.151 87.215 89.00 85.067 0.983 1.000 N.D.
±0.23 ±0.26 ±0.18 ±0.16 ±0.16 ±2.01 ±10.70 ±13.22 ±5.17 ±0.03 ±0.03
3 4.79 5.65 5.20 5.213 5.198 92.068 87.200 88.63 84.912 0.987 1.001 N.D.
±0.25 ±0.26 ±0.18 ±0.16 ±0.16 ±2.06 ±10.70 ±13.28 ±5.17 ±0.03 ±0.03
4 4.78 5.65 5.20 5.208 5.194 91.985 87.184 88.25 84.757 0.991 1.002 N.D.
±0.26 ±0.26 ±0.18 ±0.16 ±0.16 ±2.12 ±10.70 ±13.38 ±5.19 ±0.04 ±0.03
5 4.78 5.63 5.18 5.192 5.178 92.114 86.969 86.75 84.254 1.004 1.000 N.D.
±0.26 ±0.29 ±0.21 ±0.18 ±0.18 ±2.12 ±10.83 ±11.84 ±5.84 ±0.02 ±0.03
6 3.63 4.28 3.93 3.942 3.930 69.016 66.654 65.75 64.712 0.760 0.749 N.D.
±2.43 ±2.86 ±2.62 ±2.63 ±2.62 ±46.06 ±45.57 ±44.78 ±43.34 ±0.51 ±0.50
7 3.63 4.28 3.90 3.933 3.922 68.873 66.640 64.50 64.422 0.773 0.746 6.825
±2.43 ±2.86 ±2.61 ±2.62 ±2.62 ±45.97 ±45.56 ±43.56 ±43.10 ±0.52 ±0.50 ±0.57
CD5% N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S.
SE(d) 0.791 0.953 0.877 0.873 0.870 15.775 14.103 13.070 13.795 0.186 0.174
SE(m) 0.559 0.674 0.620 0.617 0.615 11.154 9.972 9.242 9.754 0.131 0.123
CV 25.104 25.545 25.562 25.408 25.401 26.129 24.533 22.585 24.548 28.414 26.421
R2 0.637 0.682 0.688 0.674 0.672 0.612 0.631 0.706 0.717 0.536 0.645

Mean values are 3 replicates

 

Table 2: Effect of packaging material (HDPE) and storage condition (refrigerator) on the physical properties of tomato (variety: Himshikhar).

Tomato: Himshikhar Storage material & condition: HDPE, Refrigerator
Days D1 (cm) D2 (cm) D3 (cm) AMD

(cm)

GMD

(cm)

Sphericity (%) Mass

(g)

Volume (ml) Surface area

(cm2)

Density

(g/cc)

Shape factor TSS

(0Brix)

1 4.28 4.98 4.80 4.68 4.673 94.006 59.219 61.25 69.171 0.971 1.027 6.200
±0.43 ±0.58 ±0.54 ±0.51 ±0.51 ±1.38 ±19.22 ±20.56 ±14.56 ±0.02 ±0.01 ±0.32
2 4.26 4.94 4.74 4.65 4.636 93.905 59.185 60.13 68.147 0.988 1.021 N.D.
±0.46 ±0.56 ±0.60 ±0.53 ±0.53 ±1.68 ±19.19 ±20.07 ±15.03 ±0.03 ±0.02
3 4.25 4.90 4.68 4.61 4.599 93.809 59.151 59.00 67.124 1.006 1.014 N.D.
±0.48 ±0.55 ±0.66 ±0.55 ±0.55 ±2.53 ±19.17 ±19.63 ±15.51 ±0.05 ±0.02
4 4.21 4.88 4.66 4.58 4.573 93.790 59.137 58.63 66.380 1.013 1.017 N.D.
±0.47 ±0.56 ±0.66 ±0.55 ±0.55 ±2.76 ±19.16 ±19.46 ±15.47 ±0.05 ±0.02
5 4.18 4.85 4.65 4.56 4.548 93.773 59.124 57.75 65.646 1.028 1.020 N.D.
±0.46 ±0.57 ±0.66 ±0.55 ±0.55 ±3.00 ±19.14 ±19.26 ±15.51 ±0.04 ±0.02
6 4.18 4.78 4.60 4.52 4.508 94.510 59.065 54.50 64.557 1.093 1.019 6.575
±0.46 ±0.63 ±0.63 ±0.56 ±0.56 ±2.00 ±19.12 ±18.56 ±15.69 ±0.06 ±0.02 ±0.43
CD5% 0.068 0.115 0.091 0.071 0.071 N.S. 0.060 1.712 1.993 0.028 N.S.
SE(d) 0.032 0.053 0.042 0.033 0.033 0.756 0.028 0.796 0.927 0.013 0.007
SE(m) 0.023 0.038 0.030 0.023 0.023 0.535 0.020 0.563 0.655 0.009 0.005
CV 1.066 1.547 1.282 1.015 1.012 1.139 0.067 1.922 1.960 1.804 1.000
R2 0.951 0.971 0.940 0.993 0.994 0.160 0.954 0.899 0.995 0.863 0.239

Mean values are 3 replicates

Table 3: Effect of packaging material (HDPE) and storage condition (BOD incubator) on the physical properties of tomato (variety: Himshikhar).

Tomato: Himshikhar Storage material & condition: HDPE, BOD
Days D1 (cm) D2 (cm) D3 (cm) AMD

(cm)

GMD

(cm)

Sphericity (%) Mass

 (g)

Volume (ml) Surface area

(cm2)

Density

(g/cc)

Shape factor TSS

(0Brix)

1 4.50 5.15 4.90 4.850 4.842 94.085 66.287 68.75 73.874 0.961 1.012 6.100
±0.26 ±0.40 ±0.36 ±0.33 ±0.33 ±1.62 ±14.76 ±13.77 ±10.05 ±0.04 ±0.01 ±0.59
2 4.50 5.13 4.86 4.829 4.822 94.180 66.185 68.38 73.263 0.965 1.008 N.D.
±0.26 ±0.43 ±0.34 ±0.34 ±0.33 ±1.92 ±14.75 ±13.79 ±10.13 ±0.03 ±0.01
3 4.50 5.10 4.83 4.808 4.801 94.279 66.082 68.00 72.651 0.969 1.005 N.D.
±0.26 ±0.46 ±0.33 ±0.34 ±0.34 ±2.29 ±14.74 ±13.83 ±10.21 ±0.03 ±0.01
4 4.50 5.06 4.83 4.796 4.790 94.731 65.917 67.50 72.298 0.975 1.008 N.D.
±0.26 ±0.45 ±0.33 ±0.34 ±0.34 ±1.97 ±14.82 ±14.39 ±10.15 ±0.02 ±0.01
5 3.45 3.88 3.68 3.667 3.662 70.941 52.855 53.75 56.310 0.737 0.752 N.D.
±2.31 ±2.61 ±2.47 ±2.46 ±2.46 ±47.31 ±37.07 ±37.69 ±38.37 ±0.49 ±0.50
6 3.43 3.85 3.68 3.650 3.645 71.125 52.760 53.50 55.789 0.739 0.756 6.475
±2.29 ±2.60 ±2.47 ±2.45 ±2.44 ±47.46 ±37.01 ±37.42 ±38.03 ±0.49 ±0.50 ±0.61
CD5% N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S.
SE(d) 0.764 0.847 0.833 0.815 0.814 17.538 9.510 9.804 11.368 0.177 0.187
SE(m) 0.540 0.599 0.589 0.576 0.575 12.401 6.725 6.932 8.039 0.125 0.132
CV 26.058 25.531 26.414 25.983 25.995 28.655 21.805 21.898 23.866 28.142 28.609
R2 0.688 0.730 0.719 0.714 0.714 0.669 0.702 0.739 0.743 0.649 0.692

Mean values are 3 replicates

Table 4: Effect of packaging material (HDPE) and storage condition (ambient temperature) on the physical properties of tomato (variety: NS-524).

Tomato: NS-524 Storage material & condition: HDPE, Ambient temperature
Days D1 (cm) D2 (cm) D3 (cm) AMD

(cm)

GMD

(cm)

Sphericity (%) Mass

 (g)

Volume (ml) Surface area

(cm2)

Density

(g/cc)

Shape factor TSS

(0Brix)

1 4.23 4.47 4.27 4.322 4.320 96.738 50.478 52.33 58.842 0.966 0.989 4.467
±0.42 ±0.35 ±0.25 ±0.33 ±0.33 ±1.10 ±10.79 ±11.68 ±9.09 ±0.01 ±0.02 ±0.06
2 4.23 4.45 4.27 4.317 4.315 96.976 50.441 51.17 58.699 0.986 0.990 ND
±0.42 ±0.35 ±0.25 ±0.33 ±0.33 ±0.69 ±10.79 ±10.80 ±9.11 ±0.02 ±0.02
3 4.23 4.43 4.27 4.311 4.310 97.219 50.405 50.00 58.555 1.007 0.991 ND
±0.42 ±0.35 ±0.25 ±0.34 ±0.34 ±0.31 ±10.79 ±10.00 ±9.14 ±0.04 ±0.02
4 4.23 4.38 4.25 4.289 4.288 97.859 50.221 48.67 57.975 1.033 0.992 ND
±0.42 ±0.38 ±0.25 ±0.34 ±0.34 ±1.30 ±10.83 ±10.07 ±9.30 ±0.07 ±0.02
5 Spoiled 5.067
6 Spoiled ±0.12
CD5% 0.396 0.341 0.240 0.321 0.320 1.046 10.281 10.298 8.722 0.052 0.021
SE(d) 0.176 0.151 0.106 0.142 0.142 0.463 4.555 4.563 3.864 0.023 0.009
SE(m) 0.124 0.107 0.075 0.101 0.100 0.328 3.221 3.226 2.732 0.016 0.007
CV 7.618 6.267 4.577 6.059 6.056 0.876 16.608 16.586 12.132 4.215 1.716
R2 0.685 0.696 0.688 0.689 0.689 0.679 0.688 0.726 0.693 0.646 0.684

Mean values are 3 replicates

Table 5: Effect of packaging material (HDPE) and storage condition (refrigerator) on the physical properties of tomato (variety: NS-524).

Tomato: NS-524 Storage material & condition: HDPE, Refrigerator
Days D1 (cm) D2 (cm) D3 (cm) AMD

(cm)

GMD

(cm)

Sphericity (%) Mass

(g)

Volume (ml) Surface area

(cm2)

Density

(g/cc)

Shape factor TSS

(0Brix)

1 4.33 4.50 4.37 4.400 4.399 97.708 50.662 51.67 60.921 0.976 0.992 5.733
±0.31 ±0.20 ±0.32 ±0.27 ±0.27 ±2.12 ±11.84 ±10.41 ±7.49 ±0.04 ±0.02 ±0.92
2 4.32 4.50 4.33 4.383 4.382 97.323 50.580 51.17 60.466 0.985 0.988 ND
±0.33 ±0.20 ±0.33 ±0.28 ±0.29 ±2.50 ±11.83 ±10.77 ±7.77 ±0.03 ±0.02
3 4.30 4.50 4.30 4.367 4.365 96.936 50.498 50.67 60.011 0.995 0.985 ND
±0.36 ±0.20 ±0.35 ±0.30 ±0.30 ±2.89 ±11.81 ±11.15 ±8.05 ±0.02 ±0.02
4 4.23 4.50 4.30 4.344 4.342 96.431 50.336 49.50 59.387 1.017 0.990 ND
±0.35 ±0.20 ±0.35 ±0.29 ±0.30 ±2.76 ±11.82 ±11.30 ±7.96 ±0.04 ±0.02
5 4.17 4.50 4.30 4.322 4.319 95.920 50.174 48.33 58.759 1.041 0.995 ND
±0.35 ±0.20 ±0.35 ±0.29 ±0.29 ±2.64 ±11.83 ±11.55 ±7.87 ±0.07 ±0.02
6 2.77 2.93 2.73 2.811 2.809 63.785 32.381 30.00 37.345 0.717 0.648 6.467
±2.42 ±2.55 ±2.39 ±2.45 ±2.45 ±55.28 ±30.33 ±27.84 ±33.21 ±0.62 ±0.56 ±0.90
CD5% N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S. N.S.
SE(d) 0.819 0.847 0.841 0.836 0.835 18.973 10.290 10.064 11.406 0.201 0.194
SE(m) 0.579 0.599 0.595 0.591 0.591 13.416 7.276 7.116 8.065 0.142 0.137
CV 24.962 24.466 25.404 24.932 24.937 25.437 26.566 26.288 24.880 25.714 25.415
R2 0.522 0.428 0.465 0.471 0.472 0.475 0.451 0.569 0.511 0.246 0.420

Mean values are 3 replicates

Table 6: Effect of packaging material (HDPE) and storage condition (BOD incubator) on the physical properties of tomato (variety: NS-524).

Tomato: NS-524Storage material & condition: HDPE, BODDaysD1 (cm)D2 (cm)D3 (cm)

AMD

(cm)

GMD

(cm)

Sphericity (%)

Mass

(g)

Volume (ml)

Surface area

(cm2)

Density

(g/cc)

Shape factor

TSS

(0Brix)

14.374.504.404.4224.42097.53752.37551.6761.5011.0180.9954.700±0.15±0.36±0.36±0.27±0.27±0.75±9.51±10.41±7.38±0.05±0.03±0.2024.324.504.374.3944.39397.72152.31349.3360.7441.0650.994ND±0.19±0.36±0.31±0.27±0.27±1.98±9.48±10.13±7.37±0.05±0.0234.274.504.334.3674.36597.09752.25147.0059.9821.1180.993ND±0.23±0.36±0.25±0.27±0.27±1.83±9.46±9.85±7.36±0.06±0.024Spoiled5.0335Spoiled±0.216Spoiled-CD5%0.2000.3640.3160.2740.2721.7229.57110.2287.4410.0550.028
SE(d)0.0890.1610.1400.1210.1210.7634.2414.5323.2970.0240.013
SE(m)0.0630.1140.0990.0860.0850.5402.9993.2052.3310.0170.009
CV5.0228.7777.8446.7606.7271.91819.85722.50213.2955.5913.102
R20.7790.7710.7760.7750.7750.7730.7720.8010.7790.7370.772

Mean values are 3 replicates

Conclusion

On the basis of the experimental finding it may be conclude that tomato variety Himshikhar packed in HDPE shows minimum shrinkage at ambient temperature then refrigerator and BOD incubator storage condition. Tomato variety NS – 524 packed in HDPE shows maximum shrinkage under refrigerator condition then BOD incubator and ambient storage condition; however samples under BOD incubator spoiled after three days and under ambient temperature storage condition spoiled after four days of storage. Tomato variety Himshikhar was found superior over tomato variety NS – 524.

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