ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Dhanya V
Assistant Professor, Department of Biotechnology, Al-Ameen College, Edathala,Aluva, Ernakulam, Kerala – 683564, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 509-516 | Article Number: 6010 | © The Author(s). 2020
Received: 26/12/2019 | Accepted: 03/02/2020 | Published: 21/02/2020

Engine oil is considered as one of the most important classes of environmental contaminant. Removal of dumped waste engine oil is a challenging task because of its persistent nature in the ecosystem. Biodegradation of used engine oil with microbial consortium offers a very promising approach in terms of cost-effectiveness and elimination of secondary pollutants. This study investigates the efficiency of a formulated bacterial consortium in engine oil biodegradation. Four novel bacterial isolates Enterobacter aerogenes, Raoultella sp, Bacillus megaterium and Bacillus cereus were selected by soil enrichment technique. The newly isolated bacterial strain was identified by 16s rDNA sequencing as Bacillus cereus strain VCRC B540. Individual cultures degrade 5 ml of used engine oil in 15 days. A polyurethane bioreactor adsorbed with the formulated bacterial consortium was very effective in degrading 75 % of used engine oil at a pH 7 within 10 days. Degradation of used engine by the bacterial strains was analyzed by Fourier Transform Infrared Spectroscopy (FT/IR). Based on the available information, this is the first report discussing the engine oil biodegradation potential of a polyurethane bioreactor and the efficiency of the formulated consortium can be used for the better removal of used engine oil from contaminated sites and different effluents.


Biodegradation, Fourier Transform Infrared Spectroscopy, Microbial consortium, Polyurethane bioreactor, Used engine oil.

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.