The present study was aimed to study the seroprevalence of Listeria monocytogenes amongst the food animals and cancer patients in and around Nagpur Region, Maharashtra, India. A total of 750 samples comprising of 600 samples from food animals and 150 samples from cancer patients were collected from various slaughter houses and Government. Hospital Nagpur, respectively. 50 samples of each meat, blood and serum was collected from 50 slaughtered goat, male cattle, buffaloes and pigs. From 50 cancer patient, each of stool, blood and serum samples were gained. Out of total sample processed 23 were found positive for Listeria spp. biochemical characterization leads 12 isolates turned out as L. monocytogenes, 01 L. seeligiri, 01 L. innocua, 01 L. grayi, 05 L. welshimeri. Human, buffalo and all blood samples except pig were found nil for the listeria while remaining showed positivity for the same. L. monocytogenes was recovered from (two isolates) beef, (four isolates) chevon and (six isolates) pig. In serodiagnosis highest seroprevalence was noted in goats (80%), followed by cattle (44%), pigs (42%) and buffalos (26%) and 26% in human cancer patients. From the research findings study focuses the prevalence of L. monocytogenes among food animals in Nagpur region of Maharashtra, India. It can be concluded that high seroprevalence is an indication for the persistence of organism in geographical area.