Research Article | Open Access
Muhammad Rizwan1, Said Amin2, Bates Kudaibergenova Malikovna3, Abdur Rauf4, Muhammad Siddique5, Kamran Ullah6, Saud Bawazeer7, Umar Farooq8, Yahia Naseer Mabkhot9,10 and Mohamed Fawzy Ramadan11,12
1Centre for Biotechnology and Microbiology, University of Swat, KPK, Pakistan.
2Department of Microbiology and Biotechnology, Abasyn University Peshawar, Pakistan.
3Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
4Department of Chemistry, University of Swabi, KPK, Pakistan.
5PCSIR Complex Peshawar, KPK, Pakistan.
6Department of Zoology, University of Swabi, KPK, Pakistan.
7Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, P.O. Box 42, Saudi Arabia.
8Department of Chemistry, University of COMSATS, Abbottabad, Pakistan.
9Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
10Research Center for Advanced Materials Sciences (RCAMS), King Khalid University, Abha-6113, Saudi Arabia.
11Deanship of Scientific Research, Umm Al-Qura University, Makkah, P.O. Box 715, Saudi Arabia.
12Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig 44519, Egypt.
J Pure Appl Microbiol. 2020;14(2):1437-1451 | Article Number: 6418 | © The Author(s). 2020
Received: 02/04/2020 | Accepted: 05/06/2020 | Published: 20/06/2020

The goals of the present study were to use silver nitrate (AgNO3) solution to synthesize plant-mediated silver nanoparticles (AgNPs) using Boerhavia procumbens extract, to evaluate the antimicrobial potential of crude B. procumbens extracts as well as the antimicrobial potential of synthesized AgNPs. The antimicrobial activity was tested against ten pathogenic bacterial strains including Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Citrobacter braakii, Providentia spp., Salmonella typhi, Salmonella para typhi, Vibrio cholera, and Proteus vulgaris and seven fungal species; Rhizopus stolonifer, Candida albican, Alternaria alternata, Aspergillus flavus, Verticillium chlamydosporium, Penicillium chrysogenum, and Aspergillus oryzae. The methanol extract was fractionated using several solvents and subjected to phytochemical analysis along with FTIR. Phytochemical analyses revealed flavonoids, tannins, saponins, steroids, quinones, and phenols in the crude plant extract. AgNPs were synthesized using B. percumbens extract and characterized by UV-Vis, Fourier-transform infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). Synthesized AgNPs were spherical, with 20-80 nm diameter. The absorption peak of synthesized AgNPs was observed at 392 nm. AgNPs have significant antimicrobial potential against selected pathogenic bacterial and fungal species as compared to different fractions of crude B. procumbens extract. The current study suggests that green synthesis is a useful technique and can be used as an alternative to antimicrobial agents against pathogenic organisms.


Boerhavia procumbens, Nyctaginaceae, scanning electron microscopy, FTIR, silver nanoparticles, Quinones


Medicinal plants have been widely used and considered the most common sources of medicine in the pharmaceutical industry (Ramadan 2008; Ramadan et al. 2008; Mohdaly et al., 2015; Abo El-Maati et al. 2016; Shakya, 2016; Rauf et al., 2018). These plants produced a wide range of bioactive compounds (Bajguz, 2007; Cushnie et al., 2014). The composition of plant secondary metabolites depends on the species, association with microbes, and soil type (Zhao et al., 2011; Morsy, 2014). These bioactive compounds have potential against a wide range of bacterial infections and the best source for the formulation of drugs, declared by the World Health Organization (Iwu et al., 1999; WHO Traditional Medicine Strategy, 2002).

Nanotechnology or nanoscale technology is a strong field of novel research dealing with design, manipulation, and synthesis of particles with 1-100 nm (Sultana et al., 2019). Nanoparticles (NPs) have several uses in cosmetics, health care, food and feed, mechanics, optics, environmental health, drug-gene delivery, biomedical sciences, electronics, space industries, chemical industries, optoelectronics, energy science, catalysis, single-electron transistors, nonlinear optical devices and photo-electrochemical applications (Iravani et al., 2014). Nanotechnology finds its importance by generating nano-products and NPs with novel and size-related physicochemical traits in contrast to bigger molecules (Chouhan, 2018).

The most important branch of nanotechnology is plant-mediated green synthesis. Due to the environmentally friendly and practical cost, it has gained more importance (Chanel et al., 2017). Green synthesis has many advantages, i.e., less toxicity, cost-effective and straightforward, less energy consumption, perform activities under moderate conditions, and combined the effect of nanoparticles and plant bioactive constituents. Furthermore, green synthesized silver nanoparticles possess more biological potential than nanoparticles synthesized by chemical methods (Choudhury et al., 2016).

Silver NPs (AgNPs) are of interest due to their novel chemical, physical, and biological traits compared to their macro-counterparts (Sultana et al., 2019). AgNPs possess distinct physicochemical properties, comprising a high thermal and electrical conductivity, surface- chemical stability, enhanced Raman scattering, catalytic activity, and non-linear optical properties (Khattak et al., 2019). Those characteristics are responsible for their role in the formation of inks, in medical imaging and microelectronics. Besides, AgNPs reveal wide-spectrum fungicidal and bactericidal potential, making them highly accessible in functional products (Tran and Le, 2013).

Boerhavia procumbens belong to the family Nyctaginaceae, nearby termed Biskhpara/Jangli, and spreading hogweed. Leaves are large, broad, and rounded beneath with a vigorous shape. Its branches (about 3 ft.) are glabrous and slender. The branches are long, glabrous, pubescent, stout, or viscous, with 1-2 inches leaves. They are oval, dull-witted, acute, and ordinarily cordate green beneath with panicle inflorescence (Fig. 1). Its habitat Africa, Pakistan, the USA, and India. In Pakistan, it could be found in Peshawar, Attock, Hazara, Sind, Baluchistan, Multan, Thal to Kurram, and Rawalpindi. Its color is purplish-red, and the blooming period is January to August (Hussain et al., 2012). Boerhavia procumbens has a lot of pharmaceutical and health-related benefits. Medicinal applications of Boerhavia procumbens include diaphoretic, diuretic, expectorant, emetic, laxative, stomachic, rejuvenates and purgative (Khalil et al., 2017; Wajid et al., 2017; Khan et al., 2020). It is mentioned to be effective against cardiac disorders, asthma, hemorrhoids, edema, eye diseases, insomnia, jaundice, kidney stone, nervous system related disorders, cough, against snake venom, urethritis, skin related diseases, intestinal, anemia, and colic kidney disorders (Hussain et al., 2012; Ali et al., 2019).

Fig. 1. Representative picture of B. procumbence

The purpose of the current investigation was to carry out green synthesize of AgNPs using aqueous extract of Boerhavia procumbens and to study the antimicrobial potential of different fractions and synthesized AgNPs against pathogenic bacteria and fungal species.

Materials and Methods

Plant collection
B. procumbence was collected from the botanical garden of the Medicinal Botanic Centre of Pakistan Council for Scientific and Industrial Research (PCSIR), Peshawar, Pakistan. The plant was identified in the Department of Botany, University of Peshawar. The leaves having a voucher name BP554 was submitted for future correspondence. The freshly collected plant was washed carefully, then air-dried for 15 days under the shed (Pirtarighat et al., 2019). The dried plant was grounded to make a coarse powder using an electric grinding machine.

Preparation of plant extract
The crude extracts were prepared with different solvents, including methanol, water, ethanol, ethyl acetate, chloroform, and n-hexane. All solvents were taken into flasks, and plant material was soaked in flasks for three days. The solvents were decanted, and the procedure was repeated four times for each solvent. All extracts were concentrated using a rotary evaporator at 45°C under vacuum. The extracts were kept in the refrigerator for further analysis (Pirtarighat et al., 2019).

Moisture determination
Dried powdered B. procumbens (5 g) was pre-weighed in the Petri dish and kept at 105-110˚C for three h. Moisture (%) was calculated regarding the air-dried sample (Pirtarighat et al., 2019).

% Moisture = [ Wight of moisture in g / Wight of plant material (g) before heating ] X 100

Determination of extractive value
The total weight of 5 g of each dried powder of plant material was extracted for about 24 h with 100 mL of respective solvent (methanol, ethanol, ethyl acetate, chloroform, and n-hexane). The flasks were shaken, and the mixture allowed to stand overnight, followed by recovery of solvents by decantation and filtration. The filtrates were dried, and the extract was weighted in percentage (Singh et al., 2013).

% Extract = [Wight of the extract (g)/ Wight of plant material (g)] X 100

Determination of ash
The total weight of 5 g of dried powder of B. procumbens was burnt at 600˚C in a furnace for three h followed by cooling. The ash was weighed, and the percentage calculated regarding the air-dried B. procumbens sample (Premnath et al., 2012).

% Ash = [ Wight of plant material after burning / Wight of plant material before burning ] X 100

Phytochemical analyses
B. Procumbens fractions were analyzed for the presence of phytochemical constituents, according to Singh et al. (2013).

Preparation of silver nanoparticles (AgNO3)
Ten g of the plant powder was boiled with 100 mL deionized distilled water. The resultant aqueous solution was filtered using Whatman filter paper three times to remove all the insoluble material. This extract was added to a 10-3 M silver nitrate (AgNO3) solution. Analytical grade AgNO3 (Molecular weight 169.87 g/mol) was purchased from Merck (Darmstadt, Germany). The solution was heated for three h in a water bath at 60°C. The solution color changed from light yellow to brown-reddish. It showed the reduction of pure Ag (I) ions. The reduced ions were monitored and measured using UV-visible spectra of the mixture of plant extract and AgNO3 solution at regular intervals. Peaks were measured on UV-Vis spectra from 200 nm and 700 nm (Shakeel et al., 2015).

Evaluation of antimicrobial activity
Antimicrobial activities were performed using well diffusion method for both B. procumbens crude extracts and AgNPs. The ethyl acetate, methanol, ethanol, water, chloroform, and n-hexane extracts of the plant and AgNPs solutions of B. procumbens were applied against ten pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Citrobacter braakii, Proteus vulgaris, Salmonella Typhi, Salmonella Para typhi, Vibrio cholerae, and Providencia stuartii)  and five fungal strains (Candida albicans, Alternaria alternata, Aspergillus flavus, Aspergillus niger, and Aspergillus oryzae). The agar media was prepared then autoclaved and dispensed into sterilized plates in Laminar flow hood (LFH) and left for the solidification. The test microorganism uniform lawn was arranged on the surface of nutrient agar media in Petri plates with the help of a sterile swab. Uniform wells of 6 mm were bored in each plate, with sterile metallic borer. Ten mg of test samples (fraction and AgNPs) were prepared in 1.0 mL of DMSO (10%) and added into particular wells. Petri dishes were incubated for 16 h at 37°C. The zones were determined after the incubation period from the width of the wells. The formula documented the percent inhibition:

% Inhibition = [  Zone of inhibition of test sample / Zone of inhibition of standard] X 100


The current study was performed to evaluate the antimicrobial potential of B. purocumbens crude extract, to synthesize AgNPs from B. purocumbens extract and to determine their biological effects against pathogenic bacteria and fungi. Leave extracts were prepared in methanol, ethyl acetate, ethanol, chloroform, n-hexane, and water. Ash (19.38%), moisture (8.2%), and extractive values were also determined (Table 1). The FTIR of all extracts was performed using FTIR Model IR Pretige-21 (Shimadzu, Japan). Different peaks of functional groups were detected in each fraction (Fig. 2-6).

Table (1):
Ash, moisture and extractive value (%) of B. procumbenc.

Ash (%) Moisture (%) Extractive value (%)
MeOH EtOH EtOAc CHCl3 n-hexane Aqueous
19.38 8.206 13 12 8 7 5 18

Fig. 2. FTIR analysis of MeOH fraction of B. percumberns

Fig. 3. FTIR analysis of EtOH fraction of B. percumberns

Fig. 4. FTIR analysis of EtOAc fraction of B. percumberns

Fig. 5. FTIR analysis of CHCl3 fraction of B. percumberns

Fig. 6. FTIR analysis of n-hexane fraction of B. percumberns

Phytochemical analysis of B. purocumbens extract
The results of the phytochemical analyses, shown in Table 2, reveal that tannins were present in methanol, ethanol, ethyl acetate, chloroform extracts. Flavonoids were absent in the n-hexane extract, while present in all other extracts. Alkaloids and terpenoids were absent in all extracts. Saponins were absent in the n-hexane extract, while present in methanol, ethanol, ethyl acetate, chloroform, and water extracts. Steroids were present is less quantity in n-hexane extract, while strongly found in all the remaining extracts. Quinones was absent in n-hexane extract, while detected in chloroform, ethyl acetate, ethanol, methanol, and aqueous extracts. Phenols were present in much higher concentration in methanol and ethanol extracts, while moderately present in n-hexane and EtOAc extracts, and least detected in chloroform and water extracts.

Table (2):
Phytochemical analysis of B. procumbenc.

S. No. Test name MeOH EtOH CHCl3 n-hexane EtOAc H2O
1 Tannins ++ +++ ++ + ++ ++
2 Flavonoids ++ ++ ++ ++ +
3 Alkaloids
4 Terpiniods
5 Saponins ++ ++ + ++ +++
6 Steroids +++ +++ +++ ++ ++
7 Quinones ++ ++ + + +
8 Phenols +++ +++ + ++ ++ +

+++: strongly, ++moderately, + weak

FTIR analysis of B. purocumbens extract
All fraction of crude extract of B. purocumbens were evaluated for the presence of functional groups using FTIR (Fig 2-7). The Fig. of each fraction was analyzed from its IR values and presented in a tabulated form (Table 5-10).

Table (3):
Zone of inhibition by B. procumbens crude extract.

Bacterial Species Zone of inhibition (mm)
H2O MeOH EtOH EtOAc CHCl3 n-hexane AgNPs Positive Control Negative Control
E. coli 09±0.38 0±0 13±0.73 12±0.44 0±0 0±0 23±0.46 16±0.94
K. pneumoniae 17±0.85 11±0.75 14±0.19 13±0.84 10±0.66 0±0 22±0.28 11±0.26
S. aureus 11±0.02 0 14±0.33 14±0.95 0±0 0±0 22±0.18 15±1.03
P.aeruginosa 0±0 12±0.66 15±0.85 14±0.28 12±0.92 0±0 19±1.03 18±0.37
C. braakii, 13±0.38 12±1.03 16±0.86 14±1.06 11±0.02 09±0.55 20±0.44 13±0.68
Providentia Spp. 10±0.28 12±0.23 15±0.90 13±0.46 09±0.28 0±0 17±0.90 18±0.39
S. typhi 15±0.55 09±0.35 12±1.00 14±0.94 09±1.04 09±0.99 20±0.23 15±1.02
S. para typhi 0±0 10±0.69 15±0 13±0.28 0±0 0±0 23±0.84 14±0.33
V. cholerae 12±0.53 0±0 13±1.02 11±0.49 0±0 0±0 15±0.92 19±0.91
P. vulgaris


09±0.18 08±0.39 14±0,29 12±0.84 0±0 07±0.18 15±0.49 13±0.25

DMSO was used as –ve while gentamicin was used as +ve control

Table (4):
Zones of inhibition of B. percumbence extracts.

Fungal Species H2O MeOH EtOH EtOAc CHCl3 n-hexane AgNPs Positive Control Negative Control
A. oryzae 13±0.23 21±0.65 17±0.24 15±0.84 13±0.84 00±00 22±0.38 14±0.48
C. albicans 10±0.39 17±0.22 20±0.84 18±0.37 16±0.58 16±0.65 20±0.74 17±0.65
A. niger 15±0.18 19±0.26 22±0.26 18±0.74 18±0.49 19±0.77 24±0.97 14±0.25
A. alternata 9±0.73 20±0.83 21±1.33 20±0.64 19±0.65 19±0.84 23±0.88 13±0.79
A.     flavus 14±0.02 17±0.99 18±0.83 14±0.29 14±0.26 14±0.26 20±0.38 17±0.84

DMSO was used as –ve while fluconazole was used as +ve control

Table (5):
FTIR analysis of MeOH fraction of B. percumberns.

IR values Bond Functional group
3340 O-H Stretch Phenols & Alcohols
2922 O-H Stretch Carboxylic acid
1722 C=O Stretch Ketones
1600 C-C=C Symmetric Alkenes
1359 N=O Bend Nitro group
1201 C-O Stretch Ethers
1116 C-O Stretch Ethers
1070 C-O Stretch Ethers
1047 C-O Stretch Ethers
1010 C-O Stretch Ethers

Table (6):
FTIR analysis of EtOH fraction of B. percumberns.

Values Bond Functional group values Bond Functional
3348 C-C=C Amines secondary 1278 C-O Stretch Ethers
2922 H-C-H Alkanes 1180 C-O Stretch Esters
2850 C-H Stretch Aldehydes 1118 C-O Stretch Ethers
1651 C=O Stretch Ketones 1072 C-O Stretch Ethers
1485 H-C-H Alkanes 1045 C-O Stretch Esters
1375 N=O Bend Nitro group 1012 C-O Stretch Ethers
1361 N=O Bend Nitro group
1319 N=O Bend Nitro group

Table (7):
FTIR analysis of EtOAc fraction of B. percumberns.

Values Bond Functional group values Bond Functional
3288 C-C=C Amines primary 1373 N=O Bend Nitro group
2918 H-C-H Alkanes 1361 N=O Bend Nitro group
2848 C-H Stretch Aldehydes 1315 N=O Bend Nitro group
1732 C=O Stretch Esters 1244 C-O Stretch Ethers
1645 C=O Stretch Ketones 1217 C-O Stretch Esters
1606 C=O Stretch Ketones 1263 C-O Stretch Ethers
1456 H-C-H Alkanes 1029 C-O Stretch Esters
1014 C-O Stretch Ethers

Table (8):
FTIR analysis of EtOAc fraction of B. percumberns.

Values Bond Functional group values Bond Functional
3346 C-C=C Amines secondary 1242 C-O Stretch Esters
2918 H-C-H Alkanes 1161 C-O Stretch Ethers
2848 C-H Stretch Aldehydes 1033 C-O Stretch Esters
1738 C=O Stretch Esters
1732 C=O Stretch Esters
1602 C=O Stretch Ketones
1558 N=O Stretch Nitro group
1539 N=O Stretch Nitro group
1456 H-C-H Alkanes
1373 N=O Bend Nitro group
1315 N=O Bend Nitro group
1269 C-O Stretch Ethers

Table (9):
FTIR analysis of CHCl3 fraction of B. percumberns.

IR values Bond Functional group
2918 O-H Stretch Carboxylic acid
1602 C-C=C Symmetric Alkenes
1375 N=O Bend Nitro group
1315 N=O Bend Nitro group
1249 C-O Stretch Ethers
1159 C-O Stretch Esters
1035 C-O Stretch Ethers

Table (10):
FTIR analysis of an aqueous extract of B. percumberns

IR values Bond Functional group
3340 O-H Stretch Phenols & Alcohols
2922 O-H Stretch Carboxylic acid
1722 C=O Stretch Ketones
1600 C-C=C Symmetric Alkenes
1359 N=O Bend Nitro group
1201 C-O Stretch Ethers
1116 C-O Stretch Ethers
1070 C-O Stretch Ethers
1047 C-O Stretch Ethers
1010 C-O Stretch Ethers

Characterization of AgNPs
The FTIR analysis was carried out to identify AgNPs associated biomolecules. The results showed that different compounds were detected in the AgNPs sample, i.e., phenols, alcohols, carboxylic acid, ketones, alkenes, nitro group, and ethers. After comparing the spectra of AgNPs and aqueous extract, it has been confirmed that AgNPs are synthesized. The differences of IR spectra of AgNPs and aqueous extract were due to the compounds of aqueous extracts that have been oxidized by silver ions. Some of the peaks present in aqueous IR spectra were absent in AgNPs extracts. This means that AgNPs have reacted with these groups (Fig. 7-8). The FTIR analysis of AgNPS was interpreted using IR values and presented in Table 11.

Fig. 7. Fourier Transformed Infrared Spectroscopy (FTIR) of aqueous extract

Fig. 8. FTIR of synthesized AgNPs

Table (11):
FTIR analysis of AgNPs of B. percumberns.

IR values Bond Functional group
3340 O-H Stretch Phenols & Alcohols
2922 O-H Stretch Carboxylic acid
1722 C=O Stretch Ketones
1600 C-C=C Symmetric Alkenes
1359 N=O Bend Nitro group
1201 C-O Stretch Ethers
1116 C-O Stretch Ethers
1070 C-O Stretch Ethers
1047 C-O Stretch Ethers
1010 C-O Stretch Ethers

pH and color
Synthesized AgNPs can also be confirmed physically by pH and color changes. AgNPs change their color from yellowish to dark brown, and pH changes from 6 to 5.5. The change in pH and color confirms the presence of AgNPs.

UV-Vis Spectroscopy of AgNPs
The UV-Vis spectroscopy is performed to characterize the AgNPs by the surface plasmon resonance (SPR) with a spectrum of 200 to 700 nm. The maximum peak absorbance with the high band was noted at 395 nm (Fig. 9).

Fig. 9. UV Spectroscopy of synthesized AgNPs

Scanning Electron Microscopy (SEM) of AgNPs
The SEM picture shows the shape and size of the AgNPs. The SEM images showed spherical shape AgNPs in scattered form focused on 0.5 µm of area, with diameter ranges from 20-80 nm (Fig. 10).

Fig. 10. Scanning electron microscope (SEM) of synthesized AgNPs

Antimicrobial activity
For antimicrobial activities, B. procumbens extracts obtained from different solvents, as well as synthesized AgNPs were screened against bacterial and fungal pathogens (Table 3 and 4). B. Percumbens plant extracts prepared using different solvents, including water, MeOH, CHCl3, EtOH, EtOAc, and n-hexane. MeOH and EtOH fractions resulted in significant activity against pathogenic bacteria and fungi. The extracts of the remaining fraction have no or very few activities. Furthermore, it was also clear that B. Percumbens synthesized AgNPs were more active than crude extracts (Tables 3 and 4). B. Percumbens synthesized AgNPs can be used to enhance the antimicrobial potential of plant constituents against a wide range of infections.


Medicinal plants have attracted significant attention to science communities due to the promising potential in life science (Antony and Sing., 2011). Pakistan is very rich in natural wealth because of it is excellent geographical condition, multiple ecological niches, and soil conditions. It is estimated that 400-600 species out of 5700 are present in Pakistan (Ahmad et al., 2007).

Nanotechnology continues to allow researchers and technologists to find new materials and essential benefits to people. However, it is also an essential part to consider the potential harmful impacts of nanoscale generated products on health as well as our environment (Hull and Bowman, 2018). Due to these facts, it is essential to determine how to decrease dangerous impacts interred by new materials. In the present study, green synthesis of AgNPs was adopted to reduce the negative impact on health and environment because this is the safest method of preparation of AgNPs. The procedure used by Ahmed et al. (2016) for the synthesis of AgNPs by the chemical method was followed. The methodology for the synthesis of AgNPs was the same, but instead of chemical synthesis, the green synthesis method was used in the present study.

The most valuable NPs for medical uses are AgNPs, which are well known for their high antimicrobial activity. AgNPs has a remarkable option for the development of bioactive and novel drugs due to their broad-spectrum activities. Silver ion is famous as a metal ion that shows antimicrobial, antifungal, and anti-algal properties. Mainly, it has been widely used as silver nitrate solution in water, which has sterilizing and also disinfecting activities (Kheybari, 2010). Hussain et al. (2012) revealed that roots containing oil of B. procumbens are composed of methyl ester derivatives of fatty acids. Eighteen types of different components were found and quantified as well. The most important and abundant was the methyl ester of linoleic acid (9%). In the present study, the phytochemical analysis revealed different types of phytochemicals, in which steroids are of great interest nowadays. B. procumbens was used as an entire plant for the process of phytochemical analysis, the plant contains eight important phytochemicals and having good antimicrobial activity. Furthermore, this ability was enhanced by synthesizing the AgNPs and transferring nanomaterials and nanoparticles through a cost-effective route.

Plants having different medically-imported bioactive components could be used to treat severe human pathogenic diseases. There are two types of categories of phytochemicals: i.e., the primary and secondary compounds. The primary compounds contain proteins, chlorophyll, sugar, and amino acids. Secondary compounds include alkaloids and terpenoids (Wadood et al., 2013). The objective of the present study was the same as both studies investigated the phytochemical profile. Important phytochemicals were identified, among them are terpenoids, flavonoids, reducing sugars, and alkaloids.

These compounds serve as a plant defense against microorganisms, insects, and herbivores. For example, phenolics are the simplest phytochemicals, which are effective against viruses (Wild, 1994), bacteria (Thomson, 1978), and fungi (Duke, 1985). Catechol and pyrogallol are both hydroxylated phenols, toxic to microorganisms. The mechanism of action is enzyme inhibition in different ways. Flavonoids are effective antimicrobial due to their ability to complex with extracellular and soluble proteins and to complex with bacterial cell walls (Tsuchiya et al. 1996). Furthermore, quinones cause inactivation of the (Kazmi et al. 1994), tannins could inactivate microbial adhesins, enzymes, cell envelope transport proteins (Nonaka et al., 1990) and alkaloids intercalate with DNA (Phillipson and O’Neil, 1987).

The clinical trial concerning the antimicrobial significance of AgNPs was carried out with the zone of inhibition produced by pathogenic organisms (gram-negative and gram-positive bacteria) and some disease-causing fungus. (Geoprincy, 2013). The present study aimed to evaluate the antimicrobial activity of B. procumbens. Among extracts used, methanol and ethanol showed significant activity against targeted pathogenic bacteria and fungi. The extract of the remaining fractions did not show or showed less activity. Interestingly, the prepared AgNPs from B. procumbens showed better results as compared to crude extracts.


The authors are grateful for the financial support provided by the Higher Education Commission of Pakistan for awarding a startup grant No (21:619/SRGP/R&D/HEC/2014. The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University for its funding this prolific research group no. (R.G.P.2.115/41).


The authors are grateful for the financial support provided by the Higher Education Commission of Pakistan for awarding a startup grant No (21:619/SRGP/R&D/HEC/2014. The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University for its funding this prolific research group no. (R.G.P.2.115/41).

The authors declare that there is no conflict of interest.

MR, SA, BKM, AR, MS, KU, SB, UM, YNM drafted the manuscript, compiled information from the literature, and designed the Fig. and tables. MR, SA, BKM, AR, MS, KU, SB, UM, YNM drafted the manuscript and gathered information from the literature. MR, SA, BKM, AR, MS, KU, SB, UM, YNM supervised, and reviewed the manuscript. MFR supervised and reviewed the manuscript.

This work was supported by the Deanship of Scientific Research at King Khalid University (Grant number R.G.P. 2/23/41/2019).

This article does not contain any studies with human participants or animals performed by any of the authors.

All datasets generated or analyzed during this study are included in the manuscript.

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