ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Syed Amir Ashraf1, Mushtaq Ahmad Khan2, Amir Mahgoub Awadelkareem1, Shaikh Tajuddin3, Md Faruque Ahmad4 and Talib Hussain5
1Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, Hail 2440, Saudi Arabia.
2MDPTU, College of Applied Medical Sciences, University of Hail, Hail 2440, Saudi Arabia.
3Department of Food Technology, FEIS, Hamdard University, Delhi – 110 062, India.
4Department of Clinical Nutrition, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia.
5Department of Pharmacology & Toxicology, College of Pharmacy, University of Hail, Hail 2440, Saudi Arabia.
J. Pure Appl. Microbiol., 2019, 13 (4): 2545-2552 | Article Number: 5864
https://doi.org/10.22207/JPAM.13.4.69 | © The Author(s). 2019
Received: 19/09/2019 | Accepted: 19/12/2019 | Published: 30/12/2019
Abstract

Food preservation and safety is drawing more attention globally due to the increasing prevalence of food-borne diseases. The natural methods of food preservation are considered safer compared to methods using synthetic preservatives. The essential oils with natural preservative properties could be useful for food safety and preservation. The objective of this study was to analyze the chemical composition of commercially available Allium sativum and Trigonella foenum-graecum essential oils by gas chromatography-mass spectroscopy (GC-MS). The antimicrobial activities of Allium sativum and Trigonella foenum-graecum essential oils were determined by agar well diffusion technique. The GC-MS analysis of Garlic essential oil (GEO) revealed that, Allyl methyl trisulfide (13.10%), Di-allyl sulfide (9.47%) and Di-allyl tetrasulfide (4.38%) were the major components, while methanolic extract of Fenugreek essential oil (FEO) showed limonene (12.92%), Silane trimethylphenyl (10.71%), carvone (4.57%) and Trigolline (0.38%) as major components. The results of our study showed a significant antimicrobial activity of GEO and FEO against the tested microbial strains, which indicates the presence of broad-spectrum antimicrobial constituents in GEO and FEO. However, further studies are needed for individual bioactive components and safety aspects for their application in food preservation.

Keywords

Bio-active components, Essential oils, GC-MS; Agar well assay; Antimicrobial activity.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.