ISSN: 0973-7510
E-ISSN: 2581-690X
In this research, the magnetic nanoparticles were synthesized by a sequential process, involving the chemical coprecipitation of Fe(II) and Fe(III) salts with ammonium hydroxide, resuspension of the nanoparticles in water, and functioned on its surface by KMnO4. The active group coated magnetic nanoparticles were loaded on Aeromonos sp.F3 to prepare the magetic Aeromonos sp.F3 which was easy to separate the enzyme from the broth for collagenase production. The magnetic nanoparticles were characterized in terms of their particle composition, size and size distribution by FT-IR spectrometer, X-ray and LPSA. The influences of the ratio of bacteria and magnetic nanoparticles, temperature, reaction time and pH on the formulation of magnetic Aeromonos sp.F3 were discussed.
Magnetic nanoparticles, Carboxyl-function, Modification, Size distribution
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