ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sajna Keeyari Purayil1, Chew Annley1, Paulraj Ponnaiah1, Sajeesh Pattammadath1, Pazhayakath Thevarkattil Mohamed Javad1, Jenifer Selvarani A.2, Raji P.2, Thirumurugan R.3, Iyappan P.1 and Antony V Samrot1
1Department of Biomedical Sciences, MAHSA University, SP 2, Bandar Saujana Putra, Jenjarom, 42610, Malaysia.
2Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiar Nagar, Chennai – 600 119, Tamil Nadu, India.
3Medical Laboratory Technologist, Department of Transfusion Medicine, JIPMER, Puducherry – 605 006, India.
J. Pure Appl. Microbiol., 2019, 13 (4): 2363-2373 | Article Number: 5952
https://doi.org/10.22207/JPAM.13.4.52 | © The Author(s). 2019
Received: 19/11/2019 | Accepted: 12/12/2019 | Published: 26/12/2019
Abstract

Five plant species namely, Phyllanthus acidus, Piper aduncum, Pandanus amaryllifolius, Macaranga peltata and Acacia mangium were analysed for their effective in-vitro bioactivity. The chloroform and aqueous extracted of the selected plants were subjected to TLC bioautography for antioxidant activity later all the extracted were subjected for DPPH assay where the chloroform extracts were found to express maximum antioxidant property. Amongst all the plants, Macaranga peltata accounted to 95% DPPH scavenging activity. The antimicrobial studies of the plant extracts were performed via agar well diffusion method, MIC determination, Biofilm inhibition assay in microtitre plate against clinical isolates like Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. It was found that Piper aduncum (chloroform and aqueous extract) and Macaranga peltata (only aqueous) expressed antibacterial activity, of which only chloroform extract of Piper aduncum could show negative influence against the biofilm development of P.aeruginosa.

Keywords

DPPH scavenging, antibacterial activity, Staphylococcus aureus and Pseudomonas aeruginosa.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.