Aim to study the whole genome of cultured and uncultured microbes present within the ant gut environment can only be determined by using the advanced technology used is Next-generation sequencing (NGS) tool. Here’ in this  research’ this tool is been used to study the exact composition or population of gut microbes present in the two ants are:  Carpenter ant (genus Camponotus) and Weaver ant (genus Oecophylla), by 16S/18S/ITS rDNA amplicon sequencing and comparing whether these two ants have same microbial species and same composition, if yes then what is their percentage of abundance in these ants gut and how these microbial diversity play role in these ants life cycle. And from this ant gut study, which is performed by metagenomic tools, revealed the presence of large  diversity of microbes in these ant gut and are from the order and genus of bacteria commonly found are Actinomycetales, Bifidobacteriales, Actinobacteria, Bacteriodales, Flavobacteriales, Caulobacterales, Methanobacteriales, Lactobacillales, Clostridiales, Bradyrhizobacterium, Agrobacterium etc. here, the  complete microbial diversity of Carpenter and Weaver ant  guts are studied by performing 16S / 18S / ITS rDNA amplicon sequencing  procedure,  which includes, surface sterilization, dissection, culturing in basic media broth, genomic DNA extraction, quality control, rDNA variable region amplification, library construction, high-throughput sequencing, data analysis and identification of microbiome. This research is done to explore the vital functions of these microorganisms in the host (Ant) and their biotechnological applications in the environment.