ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Noriko Okita1,2,5, Toshihiro Hoaki1,3, Sinya Suzuki4 and Masashi Hatamoto2
1Technology Center, Taisei Corporation, Yokohama, Kanagawa, Japan.
2Department of Civil and Environmental Engineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
3Department of Civil and Environmental Engineering, National Institute of Technology, Oita College, Oita, Japan.
4Marine Biological Research Institute of Japan Co.,Ltd
5Technology Support Center , Taisei-Yuraku Real Estate Co.,Ltd, Yokohama, Kanagawa, Japan.
J. Pure Appl. Microbiol., 2019, 13 (4): 1917-1928 | Article Number: 5785 | © The Author(s). 2019
Received: 14/08/2019 | Accepted: 20/12/2019 | Published: 26/12/2019

Phylogenetic analysis of bacteria and archaea on the seafloor surface of the Nankai Trough was conducted. DNA was extracted from a total of 14 samples (2 samples from 1 methane seep area and 12 samples from 12 general seafloor areas) and analyzed, targeting the 16S rRNA gene. As a result of the phylogenetic analysis of bacteria, 1 clone was found to have 96% homology with the 16S rRNA gene sequence of Methylomicrobium alcaliphilum, a halophilic methane-oxidizing bacterium, in a sample taken from the methane seep area. However, overall, no characteristic pattern was observed in the bacterial community structure between the methane seep area and the general seafloor. In contrast, in archaea, the genus Methanosarcina was predominantly detected in the samples from the methane seep area. Among them, many sequences that were closely related to anaerobic methane-oxidizing archaea, which perform anaerobic methane oxidation, were detected.


Methane seep, ANME, methane oxidation, MOB.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.