Multiple surveillance studies have demonstrated that resistance among prevalent pathogens is increasing at an alarming rate, leading to greater patient morbidity and mortality from nosocomial infections. Among 50 patients the isolated nosocomial pathogens are Staphylococcus sp, Streptococcus sp, Psedomonas sp, E.coli, Klebsiella sp, Enterobacter sp and Bacillus sp. Of these organisms Staphylococcus sp (35%) and Klebsiella sp (30%) are the dominant microbial pathogens. The drug resistance and sensitivity pattern of nosocomial bacterial pathogens were tested against antibiotics such as Ampicilin, Amoxycilin, Cefazolin, Gentamicin, Neomycin, Rifampicin, Tetracycline, and Vancomycin. In Staphylococcus sp shows highly resistant against Gentamicin (19mm) and Neomycin (24mm) was highly controlling and sensitivity for Rifampicin (6mm) and Vancomycin (8mm) but susceptible for Amoxycilin, Cefazolin and Tetracycline. Klebsiella show highly resistant against Cefazolin (18mm) and Neomycin (24mm) was highly controlling and sensitivity for Amoxycilin (6mm) and Vancomycin (2mm) but susceptible for Rifampicin and Tetracycline. Appropriate antibiotic selection, surveillance systems, and effective infection-control procedures are key partners in limiting antibiotic-resistant pathogen occurrence and spread.