ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
To Dinh Phuc1,2 , Nguyen Thuy Huong1, Pham Thi Thu Dan2, Bui Thi Hong Linh2 and Truong Van Mui3
1Biotechnology Department, Ho Chi Minh City University of Technology, Vietnam National University-Ho Chi Minh City (VNU-HCM), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City 700000, Vietnam.
2Biotechnology Department, Lien Hiep Phat Science Technology Company Limited, 18B Street 2, Lu Gia Housing Project, District 11, Ho Chi Minh City 700000, Vietnam.
3Biotechnology Department, Ho Chi Minh city University of Food Industry, 140 Le Trong Tan Street, Tan Phu District, Ho Chi Minh City 700000, Vietnam.
Article Number: 8635 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(3):1444-1457. https://doi.org/10.22207/JPAM.17.3.06
Received: 19 April 2023 | Accepted: 08 June 2023 | Published online: 06 July 2023
Issue online: September 2023
Abstract

The water quality including toxic gas parameters and Vibrio density is a serious problem in intensive shrimp culture. Yucca schidigera extract and Bacillus sp. are considered as a practical solution to improve the water quality. The aim of our study was to determine the effect of combination of Yucca schidigera extract and Bacillus spp. on total ammonia nitrogen (TAN) and nitrite contents, Vibrio count, and the growth performance of white leg shrimp (Penaeus vannamei). Shrimp (PL12) were assigned to seven treatments (triplicates) including treatments with combination of yucca extract and single strain of Bacillus (AY, B2Y, B3Y), combination of yucca extract and Bacillus consortium (ABBY), yucca extract only (Y), Bacillus consortium (ABB), and control treatment (C). Vibrio count was carried out by the spread plate technique every 7 days. TAN and nitrite contents were determined by using sodium nitroprusside, and sulphanilic acid with N-(1-naphthyl) ethylenediamine, respectively, at 24 hours and 168 hours after adding Bacillus strains and yucca extract. The shrimp growth parameters include the final biomass, final body weight (FBW, g), weight gain rate (WGR, %), survival rate (SR, %), and feed conversion ratio (FCR) were recorded after 5 weeks. The results showed that the water quality (0.073 – 0.179 ppm of TAN, 0.187 – 0.934 ppm of nitrite, and Vibrio count of 2.552 – 3.038 log CFU/ml) of tanks treated with combination of yucca extract and single strain of Bacillus (AY, B2Y, B3Y) or Bacillus consortium (ABBY), and Bacillus consortium (ABB) was significantly controlled compared to the control and yucca extract only treatments. The ABBY treatment most significantly improved the shrimp growth parameters (FBW, biomass, WGR, FCR, and SR – 0.424 ± 0.010 g, 40.202 ± 1.510 g, 14048.902 ± 328.756 %, 1.100 ± 0.040, and 94.667 ± 1.856 %, respectively) compared to the other treatments. Overall, our study concluded that the combination of yucca extract and Bacillus consortium could control water quality (0.036 – 0.105 ppm of TAN, 0.187 – 0.604 ppm of nitrite, and Vibrio count of 2.553 – 2.624 log CFU/ml), reduce 46.49% of FCR and significantly increase the growth performance (approximately 92.73% of FWB, 163.19% of biomass, 93.93% of WGR, 46.49% of FCR) of P. vannamei (PL12) with density of 100 individuals/100L.

Keywords

Bacillus consortium, Yucca schidigera, Ammonia Nitrite Removal, Yucca Extract, Penaeus vannamei, Shrimp Growth Performance

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.