Effect from Combinations of Yucca schidigera extract with Bacillus Strains on the Growth of White Leg Shrimp ( Penaeus vannamei ), Density of Vibrio sp., and on the Ammonia and Nitrite Content of Culture Water

The water quality including toxic gas parameters and Vibrio density is a serious problem in intensive shrimp culture. Yucca schidigera extract and Bacillus sp. are considered as a practical solution to improve the water quality. The aim of our study was to determine the effect of combination of Yucca schidigera extract and Bacillus spp. on total ammonia nitrogen (TAN) and nitrite contents, Vibrio count, and the growth performance of white leg shrimp ( Penaeus vannamei ). Shrimp (PL 12 ) were assigned to seven treatments (triplicates) including treatments with combination of yucca extract and single strain of Bacillus (AY, B2Y, B3Y), combination of yucca extract and Bacillus consortium (ABBY), yucca extract only (Y), Bacillus consortium (ABB), and control treatment (C). Vibrio count was carried out by the spread plate technique every 7 days. TAN and nitrite contents were determined by using sodium nitroprusside, and sulphanilic acid with N-(1-naphthyl) ethylenediamine, respectively, at 24 hours and 168 hours after adding Bacillus strains and yucca extract. The shrimp growth parameters include the final biomass, final body weight (FBW, g), weight gain rate (WGR, %), survival rate (SR, %), and feed conversion ratio (FCR) were recorded after 5 weeks. The results showed that the water quality (0.073 – 0.179 ppm of TAN, 0.187 – 0.934 ppm of nitrite, and Vibrio count of 2.552 – 3.038 log CFU/ml) of tanks treated with combination of yucca extract and single strain of Bacillus (AY, B2Y, B3Y) or Bacillus consortium (ABBY), and Bacillus consortium (ABB) was significantly controlled compared to the control and yucca extract only treatments. The ABBY treatment most significantly improved the shrimp growth parameters (FBW, biomass, WGR, FCR, and SR - 0.424 ± 0.010 g, 40.202 ± 1.510 g, 14048.902 ± 328.756 %, 1.100 ± 0.040, and 94.667 ± 1.856 %, respectively) compared to the other treatments. Overall, our study concluded that the combination of yucca extract and Bacillus consortium could control water quality (0.036 - 0.105 ppm of TAN, 0.187 - 0.604 ppm of nitrite, and Vibrio count of 2.553 - 2.624 log CFU/ml), reduce 46.49% of FCR and significantly increase the growth performance (approximately 92.73% of FWB, 163.19% of biomass, 93.93% of WGR, 46.49% of FCR) of P. vannamei (PL 12 ) with density of 100 individuals/100L.


INTRODUCTION
Ammonia and nitrite are major limiting factors in aquaculture systems. [1][2][3] The process of decomposition of organic compounds in the pond and from the excretory system of aquatic animals along with excess feed will produce ammonia and nitrite, when the pond accumulates a certain amount of ammonia and nitrite, the aquatic animals will be stressed and even die. 4 Feed control and daily water changes have been successfully applied to maintain acceptable ammonia and nitrite levels in the culture of Litopenaeus vannamei. 4 However, this method is extremely expensive, increases production costs, consumes labor, affects the environment and can introduce pathogens into the culture system. 3 Therefore, controlling ammonia and nitrite with an eco-friendly biological solution can be an effective alternative to large aquaculture systems.
Probiotics are also an effective solution to maintain water quality, and promote growth performance of shrimp. 5,6 Bacillus strains (Bacillus subtilis, Bacillus flexus, Bacillus licheniformis, Bacillus firmus, and Bacillus megaterium) are commonly used in aquaculture to improve water quality, especially in the treatment of ammonia and nitrite. 7,8 In addition, Bacillus sp. also has the ability to control pathogenic bacteria, including Vibrio sp. cause disease in shrimp, without causing side effects, this can replace antibiotics and bactericidal chemicals in aquaculture. 7 Bacillus consortium including Bacillus flexus QG-3, Bacillus flexus NS-4, and Bacillus licheniformis XCG-6 (proportion 5:5:4) was effectively resistant to Vibrio spp. with a inhibition ratio of approximately 90% after 144 hours in white leg shrimp (L. vannamei) culture water. 9 The bacterial consortium including Rhodopseudomonas palustris SUP-2, Bacillus subtilis SUP-3, and Bacillus firmus SUP-1 reduced the total Vibrio density by approximately 68% in the trials of white shrimp Penaeus vannamei. 6 Bacillus subtilis L10 and Bacillus subtilis G1 (proportion 1:1) decreased total Vibrio count in culture water of white shrimp L. vannamei by approximately 82 -91% after 8 weeks of the experiment. 10 One of the medicinal plants with useful uses in aquaculture is Yucca schidigera which possesses many beneficial properties for aquatic animals. The deserts of Mexico and Southwestern United States are the sources of Yucca schidigera plant. 11 In many studies, the growth performance and physiological status of aquatic animals were significantly improved when Yucca schidigera extract was added to the diet or applied directly to the aquatic environment. [11][12][13] Wang et al. studied the growth parameters of mirror carp (Cyprinus carpio) when Yucca schidigera extract was added to the diet at concentrations of 0, 200, or 400 mg/ kg for 8 weeks. The results showed that the diet containing 400 mg/kg of yucca extract significantly improved the final body weight and weight gain rate compared to the control treatment. 14 Baei et al. investigated the feed efficiency and growth of olive flounder P. olivaceus with the diet containing yucca extract (1.5 g/kg diet). The results showed that there was no significant differences between the diet supplemented with yucca extract and the control group. 15 Abdel-Tawwab et al. added yucca extract and/or yeast Saccharomyces cerevisiae to the culture water of Nile tilapia at a dose of 1 g/m 3 for 8 weeks. At the end of the experiment, the treatment with the addition of yucca extract and yeast showed the highest growth of Nile tilapia. 12 In another study, Hernandez-Acosta et al. investigated the effects of Yucca schidigera and Quillaja saponaria (NTF) extracts (0, 0.25, 0.50, 1.00 and 2.00 g/kg diet) on growth parameters of white shrimp Litopenaeus vannamei. After 40 days, the diets supplemented with 1.00 and 2.00 g/kg of NTF increased the weight and decreased FCR of shrimp compared to the control group. 16 Yucca extract improves protein metabolism leading to increased feed utilization of aquatic animals. 17 Furthermore, Yucca schidigera extract, which contains antibacterial compounds including 5b-spirostan-3b-ol, sarsasapogenin, and smilagenin, is considered antibacterial agent, enhancing the immune system for aquatic animals. 14,18,19 One of the most important functions of yucca extract is to improve water quality. Yucca extract has the ability to remove ammonia as well as reduce the adverse effects of ammonia on the health of aquatic animals due to possessing significant content of saponins and resveratrol, and can be used as a liquid or powdered supplement. 11 23 Moreover, Yucca schidigera extract has the ability to remove nitrite in aquaculture. 4 Yucca extract is one of the effective alternatives in water quality control. Therefore, yucca extract becomes an ingredient in many commercial products for aquaculture, particularly in intensive systems.
Abdel-Tawwab et al. studied the effects of Yucca schidigera extract and yeast Saccharomyces cerevisiae on growth performance of Nile tilapia Oreochromis niloticus. The results showed that 1 g/L of both yucca extract and yeast significantly reduced ammonia concentrations in the pond water (0.046 -0.055 mg/L) compared to that of the control ponds (0.124 mg/L). 12 Biernasiak et al. investigated the impact of the combination of probiotics and yucca extract to the performance and faecal microflora of broiler chicken. This study suggested that the formula containing in one kg with Lactobacillus spp. (4.0 x 10 10 CFU), Saccharomyces cerevisiae (4.0 x 10 6 CFU) and 50 grams of Yucca schidigera extract decreased Clostridium density in broilers' faeces, although there is no significant differences of FBW, FCR, BWG among treatments. This formula may be an effective alternative to antibiotic growth promoters used in poultry breeding. 24 Similar results were reported by Benamirouche et al., the diet supplemented with Pediococcus acidilactici MA18/5M (10 9 CFU/kg), Saccharomyces cerevisiae type boulardii CNCM I-1077 (10 9 CFU/kg), and Yucca schidigera extract was supplemented to the drinking water at a dose of 1L/1000L may successfully replace antibiotic in broiler production with the functions of health preservation and meat quality improvement. 25 Especially, Abdo et al. studied the improvement of water quality, histopathology, antioxidant, and innate immunity by the combination of Bacillus species and Yucca schidigera extract in Nile tilapia culture with acute ammonia exposure. The results showed that the addition of Bacillus species and yucca extract mixture to the culture water of Nile tilapia significantly controlled the ammonia concentration, restored the pH and recovered the genes relating to inflammatory and immune at the control level. 26 However, the option of combining yucca extract and Bacillus spp. (CYB) for improvement of water quality and aquatic animal's growth performance has not been widely studied. Therefore, this study was conducted to determine the effects of combining Yucca schidigera extract and Bacillus spp. on ammonia and nitrite concentration, Vibrio count in Penaeus vannamei shrimp culture water. The shrimp growth parameters in 35 days of experiment in intensive tanks were also determined.

Bacillus Isolates
Bacillus sp. strains AQ 1 , BIO 2 , and BAL 3 were isolated from sludge of Can Gio Mangrove Forest, southeast of Ho Chi Minh city, Vietnam in our previous study. The sterile vials were used for collecting the sludge samples from at least 10 cm of depth. The samples were stored in an ice box at 4°C, then transferred to the laboratory. The samples were plated onto NA media (Nutrient Agar) by spread plate method after being diluted with saline. Bacillus isolates were obtained and screened for ability of NH 3 and NO 2 removal. Bacillus sp. AQ 1 , BIO 2 , and BAL 3 have a best ability to reduce NH 3 and NO 2 among isolates. These Bacillus strains were stored at -20°C Nutrient Broth (Himedia, India) containing 15% glycerol (v/v). 27

Bacillus preparation
Bacillus strains (AQ 1 , BIO 2 , and BAL 3 ) were grown in Nutrient Broth (Himedia, India) with shaking incubator at 37°C for 24 h and centrifuged at 6000 rpm for 20 min, the supernatants were discarded. The sterile saline solution (9‰ NaCl) was used for re-suspending and washing three times the bacterial pellet. A spectrophotometer was used for measuring the density of cell suspensions at 600 nm that correlated to colony-forming units (CFU) using a spread plate technique. The bacterial suspension of Bacillus strains were stored at 4°C until use or immediately applied to the rearing tanks.

Experimental Design
The experiment was conducted at biotechnology department of Lien Hiep Phat Sci-Tech Co., Ltd (Ho Chi Minh city, Vietnam) and lasted for 35 days with 7 treatments, every treatment was triplicated. Total tank for shrimp culture is 21. Before the beginning of the trial, the shrimp were adapted to the rearing conditions for 3 days in a circular plastic tank (1000 L). The acclimatization conditions were set up similar to those in the hatchery including temperature (29°C), salinity (10%), DO (5 ppm), pH (7.8), and kH -Alkalinity (140 ppm). In this period, shrimp were fed daily with an industrial feed at 3% body weight. After adaptation, 100 shrimp were randomly reared in each 100L glass tank. The water parameters of experimental tanks were adjusted within appropriate range of white leg shrimp, as well as similar to the acclimatization stage, including kH -Alkalinity (140 -160 ppm), salinity (10%), pH ( The dosages used in the treatments were 1.5 × 10 4 CFU/ml (Bacillus spp.), 28 and 1 mg/L (Yucca extract). 12,29 Bacillus spp. and yucca extract were added to the tanks every 7 days during the experiment. Each Bacillus strain was prepared in NB at 37 o C for 24 hours. The method described in section 2.1.3 which was used for making a bacterial stock at 5 x 10 8 CFU/ml of each Bacillus strain. On the first day and every 7 days of the experiment, the bacterial stocks and yucca extract were adding into the 100L glass tanks of 6 treatments with 3 ml of AQ 1 stock and 100 mg of yucca extract, 3 ml of BIO 2 stock and 100 mg of yucca extract, 3 ml of BAL3 stock and 100 mg yucca extract, 1 ml of each bacterial stock (AQ 1 , BIO 2 , and BAL 3 ) and 100 mg yucca extract, 1 ml of each bacterial stock (AQ 1 , BIO 2 , and BAL 3 ) and none of yucca extract, and only 100 mg yucca extract to generate the final dosages in the culture water of these treatments containing 1.5 x 10 4 CFU/ml of Bacillus sp. AQ 1 and 1 mg/L of yucca extract (AY), 1.5 x 10 4 CFU/ml of Bacillus sp. BIO 2 and 1 mg/L of yucca extract (B 2 Y), 1.5 x 10 4 CFU/ml of Bacillus sp. BAL 3 and 1 mg/L of yucca extract (B3Y), 1.5 x 10 4 CFU/ml of Bacillus sp. AQ 1 , BIO 2 , BAL 3 and 1 mg/L of yucca extract (ABBY), 1.5 x 10 4 CFU/ml of Bacillus sp. AQ 1 , BIO 2 , BAL 3 (ABB), and 1 mg/L of yucca extract (Y), respectively. The tanks of control treatment were not supplemented with Bacillus strains and yucca extract.

Nitrite determination
Nitrite concentration was determined using APHA 9245 B -Multiple tube method. 1 ml of sulfanilic acid (1% v/v), 1 ml of N-(1-Naphthyl)ethylenediamine dihydroclorua (0.1 % w/v) were added into the tube contained 5 ml of sample. UV/VIS Spectrophotometer (LLG -uniSPEC 2, Germany) was used for measuring this solution at 540 nm absorbance after incubation at least 30 minutes in dark. 33

Determination of Bacillus and Vibrio density
Water samples were collected every 7 days during the experiment. The enumeration of bacteria was carried out by the spread plate technique. Saline (0.9% NaCl) was used to dilute the water samples for determining total plate count (TPC) on trypticase soy agar (TSA), Vibrio count (VC) on thiosulfate citrate bile salt sucrose agar (TCBS), Bacillus sp. (BS) on nutrient agar (NA) via spore count. 34

Statistical analysis
One-way analysis of variance (ANOVA) was used to analyze all data of experiments that determine the statistically significant differences among the treatments (Duncan's test) by Statgraphics Centurion XV software.

Effect of CYB on ammonia and nitrite content of culture water
The obtained results showed that CYB was effective in controlling TAN and nitrite in shrimp culture water (Figure 1 and 2). The TAN concentrations of ABB, AY, B2Y, and B3Y treatments increased in the first week, then gradually decreased from the second week until the end of the experiment. The TAN content in the water of the Y treatment was well controlled within 3 weeks (D0 -D22), and increased in the remaining 2 weeks. At the end of the experiment, the TAN concentration in the water treated with ABBY, ABB, AY, B2Y and B3Y had no statistical difference and was significantly lower than compared to the treatment supplemented with only yucca extract (Y) and the control with ppm values of 0.073 ± 0.011 a , 0.179 ± 0.011 a , 0.079 ± 0.015 a , 0.093 ± 0.018 a , 0.080 ± 0.014 a , 0.708 ± 0.050 b , and 5,820 ± 0.107 c , respectively.
The nitrite concentration in the water treated with only yucca extract at all sampling times was significantly lower than the control but higher than the treatments supplemented with Bacillus strains. The ABBY treatment showed the most effective control of nitrite content compared to the other treatments throughout the experiment. The AY, B2Y, and B3Y treatments needed 4 weeks to stabilize nitrite content in shrimp culture water. The ABB and Y treatments did not stably control the nitrite content of the water during the trial. At the end of the experiment, the lowest content

Effect of CYB on total Vibrio count in culture water
The initial TPC among the treatments was not statistically different, the density fluctuated in the range of 4.061 -4.158 log CFU/ml. The significant differences of TPC were obtained at weeks 1 to 5, especially at week 5, where TPC of ABBY, ABB, AY, B2Y, and B3Y treatments was significantly higher compared to the others with log CFU/mL values of 5.331 ± 0.019 b (Control), 5.771 ± 0.009 c (ABBY), 5.767 ± 0.007 c (ABB), 5.282 ± 0.018 a (Y), 5.773 ± 0.011 c (AY), 5.763 ± 0.005 c (B2Y), and 5.781 ± 0.007 c (B3Y). The results showed that the treatments supplemented with Bacillus strains had a shorter time to increase TPC compared to the control and the treatment only supplemented with yucca extract (Figure 3). Figure 4 showed that the Bacillus density of the control and Y treatment had no statistical difference, only slightly increased after 2 weeks and almost unchanged until the end of the experiment. However, the Bacillus density of the remaining treatments increased obviously after the first week, and there was no statistical difference among treatments during the experimental period. The ABBY, ABB, AY, B2Y, and B3Y treatments had the highest Bacillus counts at the end of the experiment which was significantly different from the Bacillus counts of the control and Y treatment, with log CFU/ml values of 4.040 ± 0.022 b , 4.023 ± 0.008 b , 4.038 ± 0.016 b , 4.010 ± 0.005 b , 4.028 ± 0.009 b , 2.564 ± 0.017 a and 2.605 ± 0.013 a , respectively. Thus, strains of Bacillus sp. AQ 1 , BIO 2 , BAL 3 all grow and maintain high density in shrimp culture water.
The initial Vibrio count among the treatments had log CFU/ml values in the range of 2.389 -2.542, that were not significantly different. After week 1, Vibrio count tended to increase in all treatments. In the treatments supplemented with Bacillus and yucca extract, the Vibrio density was

Effect of CYB on ammonia and nitrite content of culture water
The combination of two different agents including Bacillus sp. and Yucca schidigera extract in controlling water quality and improving growth parameters of P. vannamei shrimp has not yet been studied. The results of this study showed that the combination of Bacillus spp. and yucca extract were significantly better than either single agent. Treatment of ABBY, AY, B2Y, and B3Y with 1 mg/L of yucca extract and 1.

Effect of CYB on the growth performance of shrimp
Bacillus strains and yucca extracts had a positive effect on the growth parameters of shrimp (Table). The final body weight, biomass, weight gain rate, survival rate, and FCR of the treatments supplemented with Bacillus strains and yucca extract were all significantly better than the control treatment.
The FCR of shrimp from ABBY and AY treatments were significantly lower (1.100 ± 0.040 a , and 1.089 ± 0.020 a , respectively) than that of shrimp from the other treatments with values of 2.056 ± 0.034 c (Control), 1.265 ± 0.038 b (ABB), 1.317 ± 0.022 b (Y), 1.231 ± 0.039 b (B2Y), 1.294 ± 0.027 b (B3Y). The survival rate of the treatments supplemented with Bacillus strains and yucca extract was not significantly different ranging from 88 to 94%. The weight gain rate (WGR) of ABBY treatment was the highest and was significantly different from all other treatments. In the treatment only adding yucca extract, WGR was higher than that of the control but lower than that of all other treatments. FWB and biomass of ABBY treatment were significantly higher than that of the other treatments (Table).
saponin and glycocomponent fraction that can bind ammonia or convert ammonia into nitrite and nitrate. 18 On the other hand, Bacillus sp. was considered as one of the probiotic strains commonly used for controlling ammonia and nitrite in aquaculture. 2 Bacillus spp. likely possess the hao gene encoding the enzyme hydroxylamine oxidoreductase, and nxrB gene encoding the enzyme nitrite oxidoreductase, which is involved in the oxidation of ammonia and nitrite. 36 This made the reduction of ammonia and nitrite more powerful when the yucca extract was combined with Bacillus strains.

Effect of CYB on total Vibrio count in culture water
In the present study, Bacillus counts of ABBY, AY, B2Y, and B3Y treatments were much higher than those of the control and the treatment with yucca extract only. This shows that the Bacillus strains added to the shrimp tank grew well until the end of the experiment. In addition, the tanks were supplemented with yucca and Bacillus spp. will control Vibrio sp. better than the addition of yucca extract. The ABBY and ABB treatments had a Bacillus consortium (AQ 1 , BIO 2 , and BAL 3 ) have outstanding Vibrio control. Bacillus sp. can control pathogenic bacteria in ponds with antibacterial compounds and quorum-quenching enzymes. 37 Bacillus species produce more than 800 different peptide antibiotics, which are antibacterial compounds that effectively against bacteria [38]. Some antibacterial peptides derived from Bacillus spp. including bacitracin (Bacillus megaterium, Bacillus subtilis, and Bacillus licheniformis), lichenin (Bacillus licheniformis), megacin (Bacillus megaterium), coagulin (Bacillus coagulans), polyfermenticin (Bacillus polyfermenticus), cerein (Bacillus cereus), thuricins, tochicin, kurstakin, entomocin and bacthuricin (Bacillus thuringiensis). [39][40][41] The chemical signal molecules (autoinducers) were produced and released from quorum sensing bacteria in response to change the cell populatin density. 42 While there are many types of signaling molecules produced by bacteria, Autoinducer-1 (AHLs -N-acyl homoserine lactones) derived from gram-negative bacteria is perhaps the most interesting. 43 Marine Vibrio is a common genus of bacteria that produces AHLs signaling molecules. 44 Quorum quenching implies degradation of autoinducer signaling molecules by the enzymes AHL lactonase, AHL acylase, and AHL oxidoreductase. 45 Bacillus species (Bacillus cereus, Figure 5. Vibrio density in water samples of treatments during experimental period Bacillus mycoides, and Bacillus thuringiensis) are AHL-degrading bacteria that produce AHL lactonase enzymes, which hydrolyze the lactone ring of AHLs to acylated homoserine. 46,47 Bacillus cereus LNE7 was shown to degrade the signaling molecule (AHL N-3-hydroxybutanoyl--homoserine lactone) of Vibrio campbellii. 48 Bacillus thuringiensis and Bacillus cereus isolated from barramundi fish Lates calcarifer identified as AHL-degrading bacteria which produce AHL lactonase enzymes (AiiA group). These two strains are resistant to Vibrio haveryi and Vibrio alginolyticus through degradation of two signaling molecules N-[(RS)-3-Hydroxybutyryl]--homoserine lactone and N-(3-Oxodecanoyl)--homoserine lactone. 43 Bacillus licheniformis DAHB1 was determined to inhibit Vibrio parahaemolyticus DAHP1-GFP through quorum quenching with the enzyme AHL lactonase. 49 Furthermore, Bacillus strains have the ability to prevent the dominance of other microorganisms including pathogenic microorganisms, helping to maintain a stable microbiome in ponds. 8 Previous studies also reported that the consortium of microorganisms and the Bacillus consortium were able to antagonize or inhibit very effectively Vibrio spp. in shrimp culture. 6 50 In another study, Zhang et al. reported that the combination of Bacillus consortium (Bacillus subtilis and Bacillus licheniformis) and isomaltooligosaccharide (IMO) added to the diet at 10 8 CFU/g and 0.2%, respectively, reduced the total Vibrio density in the shrimp gut and improved survival rate of shrimp challenged with Vibrio alginolyticus. 51 The correlation between the TPC and total Vibrio count among treatments (Figures 3 and 5) showed that Vibrio sp. in the control treatment was the predominant bacterial strain with 4.146 ± 0.022 d log CFU/ml (approximately 1,504 x 10 4 CFU/ml) while the total Vibrio count exceeding 10 4 CFU/ ml of pond water can cause diseases in shrimp. 52

Effect of CYB on the growth performance of shrimp
Nitrite and TAN concentrations in the water of the control treatment increased over time compared to the other treatments (Figure 1 and 2). This is probably the reason why shrimp growth parameters (FBW, biomass, WGR, and FCR) of the control treatment were much worse than that of the other treatments, especially the survival rate with 40,333 ± 8.819 a % (Table). In contrast, the ABBY treatment with the combination of Bacillus consortium and the yucca extract showed better growth parameters than the other treatments. This is similar to the study of Abdel-Tawwab et al. when the addition of a combination of Saccharomyces cerevisiae and yucca to the pond water increased the growth parameters of Nile tilapia Oreochromis niloticus better than using either yucca extract or yeast Saccharomyces cerevisiae. 12 Bacillus cereus and Pediococcus acidilactici probiotics, which were added to ponds water, improved FCR and weight gain of white leg shrimp (Penaeus vannamei). 53 In addition, Barman et al. also tested the consortium of Rhodopseudomonas palustris SUP-2, Bacillus subtilis SUP-3, and Bacillus firmus SUP-1 in the culture of white shrimp Penaeus vannamei, the results showed that the bacterial consortium significantly promoted the shrimp growth parameter. 37 Previous studies have suggested that extracellular enzymes biosynthesized from Bacillus spp. such as protease, lipase and carbohydrolase promote better shrimp growth and improve pond water quality. 5,9 Besides, yucca extract in the previous study has also shown the ability to promote the growth of white leg shrimp higher than the control with 9.76% (FBW -Final body weight), 9.72% (PWG -Percentage weight gain), and 20.81% (Biomass). 4 Although the mechanism of yucca extract's growth-promoting is still unclear, steroidal saponins and other bioactive compounds of Yucca schidigera may cause the cell membrane structure of the animal's gastrointestinal epithelium changes in a positive way, 54 helping to better absorb nutrients. 4,31 Some other theories suggest that yucca extract stimulates animal biosynthesis of digestive enzymes, enhances metabolism, and increases nutrient absorption. 4,55 Bacillus consortium combined with yucca extract efficiently improved the water quality by controlling TAN and nitrite concentrations, and significantly inhibited Vibrio spp that enhances surviral rate of shrimp. This combination also effectively enhanced the FBW, biomass, WGR, and FCR of Penaeus vannamei shrimp that was better than either Bacillus sp. or yucca extract.

CONCLUSION
The present study showed that the combination of yucca extract (1 mg/L) and Bacillus consortium (1.5 x 10 4 CFU/ml) could effectively reduce 46.49% of FCR, promote shrimp growth parameters higher than compared to the control with approximately 92.73% (FBW), 163.19% (Biomass), and 93.93% (WGR), exhibit efficient control of Vibrio sp., ammonia, and nitrite with values of 2.553 log CFU/ml, 0.073 ppm, 0.187 ppm compared to control with value of 4.146 log CFU/ ml, 5.820 ppm, 6.719 ppm, respectively, in the white leg shrimp rearing water. This combination as water additives was suitable for adding to the rearing water of P. vannamei (PL 12 ) with density of 100 individuals/100L.