ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Kolli Guna Ranjan1 , Girija Sankar G.2, and D.V.V. Satyanarayana Raju3
1GITAM Institute of Pharmacy, GITAM University, Visakhapatnam – 530 017, Andhra Pradesh, India.
2College of Pharmaceutical Science, Andhra University, Visakhapatnam -530 003, Andhra Pradesh, India.
3FOR U International Private Limited, East point colony, Visakhapatnam – 530 003, Andhra Pradesh, India.
J Pure Appl Microbiol. 2020;14(3):2085-2091 | Article Number: 6524 | © The Author(s). 2020
Received: 09/07/2020 | Accepted: 24/08/2020 | Published: 07/09/2020

There is increasing scientific evidence and commercial interest for using probiotics for eliminating and handling of specific diseases. Probiotics can be evaluated for its role and performance against isolated pathogens from contaminating sources. The present work reports on invitro antimicrobial activity of commercial selected probiotics against pathogenic microbe Vibrio parahaemolyticus. The work also describes cytotoxic activities using MTT assay and adherence studies of selected probiotics. Results for the studies showed maximum zone of inhibition 13.66±0.46mm in probiotic enteroplus,12.33±0.93mm in lactobacillus (NCIM2056) and 10.66±0.93mm in Avant Bact. Cytotoxicity was expressed as IC50(µg/ml) values, observed on CaCO cell lines for different probiotics. Avant Bact showed a IC50 value of 104.7745, Lactobacillus (NCIM2056) a value of 58.13223 and Enteroplus a value of 50.09716. These values expressed different safety aspects of probiotics used for study. Finally the adherence study was done to check probiotic colonizing capacity. The probiotics showed varied adherence capacity against caco cell lines. Enteroplus has % adhesion of 10.25±0.74, Avant Bact. 7.25±0.82 and Lactobacillus (NCIM2056) 7.5±1.12. In conclusion antimicrobial results show importance of probiotics to be used against specific gastro intestinal diseases. Cytotoxicity determines safety aspects of probiotics and adherence study determines probiotic as a promising candidate for in vivo studies.


Vibrio parahaemolyticus, Enteroplus, Avant Bact., antimicrobial, cytotoxicity, adherence

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