The resin extract of Commiphora myrrh is Widely used in the folk medicine. The studying myrrh resin extract include moisture. minerals such as (Ca, Fe, Mg, Na, Cu and Zn), protein, total fat and crude fiber. In this study used Muffle furnace, Kjeldahl methods Soxlet and atomic absorption. HPLC using to evaluating Polyphenol constituents of myrrh different resin extract (ethanol, ethyl acetate, petroleum ether and chloroform) as Conc. (µg / g) and in all extract (ethanol, ethyl acetate and petroleum ether and chloroform) it contained Chlorogenic acid, gallic acid Catechin, Coffeic acid, caffeine, Syringic acid, Coumaric acid, Ferulic acid, Naringenin, 4`.7-Dihydroxyisoflavone, Cinnamic, Propyl Gallate Vanillin, Querectin and Acid Ellagic acid in different concentration percentage and area The effect of Commiphora myrrh (ethanol, ethyl acetate, petroleum ether and chloroform) resin extract against four different pathogenic bacteria Salmonella typhimurium, Pseudomona aeruginosa, Escherichia coli, and Bacillus cereus, were examine by Mueller Hinton Agar and measuring inhibition zone (diameter mm), show that there were significant different among bacteria and different method of extract. All different Commiphora myrrh seed extract (aqueous, ethyl acetate and petroleum ether) have high activity against Candida albicans fungus. The study was conducted to identified the Commiphora myrrh nutritive value, polyphenol Compound and the activity against bacteria and fungi.