Mycetoma is a chronic slowly progressive localized subcutaneous infection caused by actinomycetes or fungi and characterized by formation of micro abscesses discharging grains containing aggregates of the causative agents which are discharged onto the skin surface through multiple sinuses.¹ The granules in mycetoma are mostly black, yellow and white.²

In India, actinomycotic mycetoma is prevalent in south India, southeast Rajasthan and Chandigarh; while eumycetoma is mainly reported from north India and central Rajasthan.³ Here, we report an unusual rapidly progressive eumycetoma in an infant.

Case history
A nine month old male baby, presented with multiple non-healing discharging sinuses (Figure 1) in the left lower limb of three month duration. The lesion started with a small swelling in the left lower limb. Within one month, it had grown in size with development of small ulcers turning in to multiple discharging sinuses. The patient had low-grade intermittent fever. The parents of the patient could not recollect any history of trauma. A provisional diagnosis of osteomyelitis was made.

Before investigating the case, a written informed consent was obtained from the parents of the patient. Examination revealed swelling of affected lower limb with multiple discharging sinuses covered with slough with deep induration involving an area of 3.5 X 2.5 inches. The sinuses were fixed to deeper structures discharging purulent fluid with white granules. There was no regional lymphadenopathy. Laboratory investigation revealed low hemoglobin (7.6 g/dl), normocytic, normochromic anemia and a high total leucocyte count (23.32 × 10³/µl). Mantoux and Venereal Disease Research Laboratory (VDRL) tests were negative. Chest X-ray was within normal limit. X-ray (left leg) showed soft tissue swelling with obliteration of fascial planes (Figure 2). Radiologically there was no evidence of osteomyelitis.

After thorough cleaning of the lesions, a pasteur pipette was introduced in to the sinuses. The aspirated material was rinsed in sterile saline and allowed to settle. A few white, irregular granules of variable size were observed in the aspirate and discharges. (Figure 3) The granules were crushed and subjected to microscopy and culture.⁴ Biopsy tissue from the sinus was also collected.

Gram stain, potassium hydroxide (KOH) mount, Ziehl-Neelsen stain and modified Ziehl-Neelsen stain (1% H₂SO₄) were done. The specimens were inoculated in to blood agar and MacConkey agar for bacterial culture and incubated at 37^oC for 3days. The granules were also inoculated onto two sets of Sabouraud’s dextrose agar with chloramphenicol (0.05 mg/ml) (SDA-C) and incubated at 30°C and 37°C respectively. These were also inoculated onto two sets of brain heart infusion agar with 5% sheep blood and were incubated at 37°C for 14 days under aerobic and anaerobic conditions. The specimens were also processed to exclude tubercular etiology by standard methods.

The microscopic examination of KOH mount of the aspirate and biopsy tissue showed septate hyaline hyphae and spores. (Figure 4A & B) Gram staining was performed to distinguish between actinomycetoma and eumycetoma which showed gram negative septate hyphae. (Figure 5) Histopathological examination of the hematoxylin-eosin stained tissue revealed nonspecific granulomatous inflammation with a central focus of acute inflammatory reaction surrounding grains in the subcutaneous tissue which was surrounded by histiocytes and a mixed inflammatory infiltrate comprising lymphocytes, plasma cells, eosinophils, macrophages and fibrosis along with thick club shaped structures and septate fungal hyphae.

Yellow to green velvety colonies appeared on SDA-C plates after 4 days. Microscopic morphology of the colony showed conidiophores with single phialides covering approximately the entire vesicle which was compatible with that of Aspergillus flavus.. (Figure 6A & B). Repeatedly three different samples were tested and on each occasion Aspergillus flavus was isolated. Bacteriological cultures were sterile.

The antifungal susceptibility test done at Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh by CLSI M38-A2 protocol showed the MIC of amphotericin B, fluconazole, voriconazole, itraconazole, posaconazole, caspofungin, anidulofungin and micafungin to be 0.5µg/ml, 32 µg/ml, 0.5 µg/ml, 0.06 µg/ml, 0.06 µg/ml, 32 µg/ml, 16 µg/ml and 16 µg/ml respectively.⁵  Surgical curettage and debridement were done along with treatment with itraconazole (5mg/kg/day) for 8 weeks. The patient responded to treatment with regression of swelling after two months without any discharge. No relapse was seen on subsequent follow up for 6 months. (Figure 7).