Pseudomonas aeruginosa (P. aeruginosa) is one of the common pathogen that   causes serious infections in hospitalized patients throughout the world. It  has  been  reported  that the  clinical  isolates of P. aeruginosa  are  difficult  to  treat  because of  their  virulence factors  and  antibiotics  resistances. The aim of present study was  to  determine  whether  a  correlation exists   between  the  prevalence  of   virulence  factors including   lasB , lasA  ,  PopB,  toxA  and  antibiotic  resistance  in  P. aeruginosa  isolated  from different  wards of  hospitalized  patients in Northwest of Iran. In this study, 150 isolates of P. aeruginosa were collected from the wound, UTI, LRT, sputum, burn and blood stream infections. The prevalence of toxA, lasA, lasB and PopB genes was determined by PCR. Antimicrobial susceptibility testing was performed using the kirby-bauer method. Prevalence of the isolates encoding exotoxin A was 87.33 %, lasA was 30 %, lsaB was 46.66 % and PopB was 28.66%. Prevalence of lasB gene was significantly higher in isolates from blood and respiratory tract infection in comparing with isolates from wound infections. High resistance levels to Gatifloxacin (81.33 %), Piperacillin (71.33 %), gentamicin (69.33%) and Ciprofloxacin (64%) were observed. Colistin and Polymyxin B were the most effective antibiotics. findings of the present study showed type II secretion toxin, toxA, lasA and lasB were predominant in P. aeruginosa infections from our region. Prevalence of the PopB gene was significantly lower than other previous studies. The high antibiotic resistances against antimicrobial agents were observed except for colistin and Polymyxin B which shows priority needs for developing antibiotic stewardship in our regional hospitals.