ISSN: 0973-7510

E-ISSN: 2581-690X

Mohamed Mokhtar Mohamadein1 , Rasha Mohamed Farrag2 and Eman Ahmed Helmy3
1Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt.
2Faculty of Applied Medical Science, King Salman Ben AbdelAziz University, Al-Kharj, KSA.
3Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt.
J Pure Appl Microbiol. 2012;6(4):1531-1540
© The Author(s). 2012
Received: 18/09/2012 | Accepted: 23/11/2012 | Published: 31/12/2012
Abstract

Sequencing of the conserved region of ver1 gene in each of two Egyptian Aspergillus sp., A. nidulans and A. parasiticus isolates, was conducted. Variation in DNA size (515 bp for A. nidulans and 495 bp for A. parasiticus) was observed with a high homology degree of 97%, however with no difference in the translated amino acid sequence. The obtained ver-1 gene sequence of both isolates was matched, using genebank database, with that of each of a no. of strains belonging to Aspergillus species, protein homology with the most similar strain was then conducted. No difference in the translated amino acids was observed for ver-1 gene of the A. nidulans isolate while two amino acid variations were observed in case of A. parasiticus isolate. The obtained sequences of the two investigated local isolates revealed no matching results with any of the A. nidulans strains on the gene bank database reflecting the uniqueness of the A. nidulans Egyptian isolate.

Keywords

Aspergillus sp., Conserved region, Ver-1 gene, Homology

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.