An ultra-high-performance liquid chromatograph-tandem mass spectrometry (UPLC/MS/MS) method was developed for the determination of amoxicillin sodium and tylosin tartrate in swine plasma. Amoxicillin sodium and tylosin tartrate were orally administrated to swine, and the pharmacokinetics were evaluated in plasma samples by UPLC/MS/MS after solid phase extraction. The linear range for amoxicillin sodium was 0.06–1 ¼Àg/mL (r2 = 0.9975) and that for tylosin tartrate was 0.5–50 ng/mL (r2 = 0.9994). The limit of quantitation and limit of detection for amoxicillin sodium were 0.2 ¼Àg/mL and 0.06 ng/mL, respectively, and the corresponding values for tylosin tartrate were 1.7 ng/mL and 0.5 ng/mL. The intra- and inter-day coefficients of variation were <9.8 % for amoxicillin sodium and <13.5 % for tylosin tartrate. The absolute recoveries of amoxicillin sodium and tylosin tartrate from swine plasma were 67 % and 72 %, respectively. The method showed excellent specificity, accuracy, precision, recovery, and stability. The pharmacokinetics of amoxicillin sodium (15 mg/kg) and tylosin tartrate (10 mg/kg) in healthy swine after administration of a single or compound dose were evaluated. Tylosin influenced the pharmacokinetics of amoxicillin, but amoxicillin did not affect tylosin.
UPLC/MS/MS, Pharmacokinetics, Amoxicillin sodium, Tylosin tartrate, Compound Amoxicillin and Tylosin
Share This Article
© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.