ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Muslim Suardi1,2, Annisa Suri Hamdani1, Bertiliyus Ariyati1, Rika Sari Lalfari3, Dede Lutfrian1, Asiska Permata Dewi4, Rizka Permata Suci5 and Akmal Djamaan1
1Faculty of Pharmacy, University of Andalas, Padang Indonesia.
2Department of Pharmacy, University Mohammad Natsir, Bukittinggi, Indonesia.
3Department of Chemistry, Faculty of Sciences, University of Andalas, Padang, Indonesia.
4Department of Pharmacy, Faculty of Medicine and Public Health, Abdurrab University, Pekanbaru, Indonesia.
5Department of Chemistry, Faculty of Sciences, University of Indonesia, Jakarta, Indonesia.
J Pure Appl Microbiol. 2018;12(3):1163-1169 | © The Author(s). 2018
Received: 17/06/2018 | Accepted: 13/08/2018 | Published: 30/09/2018

Utilization of rice straw (Oryza sativa Linn) as a raw material in the production of poly(3-hydroxybutyrate), P(3HB), biopolymer by fermentation using Pseudomonas aeruginosa has been conducted. Rice straw stew and microcrystalline cellulose obtained from rice straw were used as carbon source for bacterial growth at various concentrations. The content of P(3HB) in the biomass was determined by gas chromatography using the RTX-1 column and the FID detector. Results showed that the best condition in producing P(3HB) was using 1% microcrystalline cellulose as carbon source and duration of fermentation of 48 hours. The biomass and P(3HB) obtained from MCC were  982 mg/100 mL and  17.19% w/w, respectively. While using rice straw stew were 269 mg/100 mL and 1.73%, respectively.  It can be concluded that the highest P(3HB) produced was found using microcrystalline cellulose obtained from rice straw waste as a carbon source.


Rice straw, poly(3-hydroxybutyrate), Pseudomonas aeruginosa, Microcrystalline cellulose

Article Metrics

Article View: 1043

Share This Article

© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.