Abiotic stress factors are the main limitations to plant growth and yield in agriculture. Among them, drought stress, which is caused by water deficit, is probably the most impacting adverse condition and the most widely encounter Rice (Oryza Sativa L.) is one of the most important cereal crops that provides a staple diet for almost half of the world’s population. However, rice yield and quality are affected by environmental stress. Drought is one of the most common environmental stresses affecting rice growth and productivity. There’s the possible development of biotechnological tools to address the critical problems of crop improvement for a sustainable agriculture. Among the available biotechnological tools, the in-vitro culture alone or combined with mutagenesis, which are induced with physicochemical or biological agents by using (PEG) can be exploited to increase genetic variability and mutants, as a potential source of new commercial cultivars, in-vitro culture environments . Following this, it is preferred that there are more studies on finding the varieties of rice tolerant to drought stress by using biotechnological in order to reduce the risk of abiotic stress, which is the most important stresses of drought on agriculture and rice production.