By observing the effect of pH and temperature on mycelia growth of eight ECM fungal species from larch (Larixgmelini and L. kaempferi), we found that only Boleteuspictus and Suillus grevillei-2 with maximum optimal growth of pH 4.0 and of 25 °C belong to the species of narrow range of pH and temperature, while other species varied from pH 4.0 to pH 8.0, and at 10~30°C. Furthermore,all larch species contained four main lipid biomarkers of 18:2w6,9c, 18:1w9c, 16:0and 10Me 18:0 TBSA, accounting for up to 80% of relative abundance. The values of 18:1w9c / 16:0, 16:0/10Me 16:0 and 18:2w6,9c/TBSA 10Me 18:0 were 1: 1 in all the ECM mycelia, and 5: 1 in limit to ECM mycelia of larch in terms of fatty acids stoichiometry. By multivariate analysis, the fatty acids profiles had clearly separated either between species in larch and in Norway spruce, or between S. grevillei-1 in L. gmeliniand S. grevillei-2 in L. kaempferi.Taken together, these findings suggested that FA profiles of pure cultured mycelia might have a chemotaxonomic potential in ECM fungi, and that FA composition as well as FA stoichiometry were affected by the host tree species and environmental factors.