Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by consuming contaminated meat or by contact with cat feces containing oocysts. In adolescence and adulthood, most infections are subclinical. However, if a pregnant woman contracts toxoplasmosis, it may be passed through the placenta to the fetus, resulting in congenital toxoplasmosis, which is a cause of mortality and malformation. Asymptomatic infants may develop anomalies later in life.The objective of this study was to determine the seroprevalence of Toxoplasma gondii infections. Samples were collected about 103 samples from Arogya Niketan obstetric clinic, Lukerganj in Allahabad over period of one year, from November 2012 to November 2013, to estimate seroprevalence of IgM and IgG antibodies to Toxoplasma gondii. Serum samples were collected and assayed quantitatively by using automated ELISA technique. Out of 103 serum sample, 26(25.2%) and 02(1.9%) were positive for Toxoplasma IgG and Toxoplasma IgM respectively. Toxoplasma IgM was in first trimester 01(5.3%) and second trimester 01(1.7%) while Toxoplasma IgG was seen 03(15.8%), 15(25.4%), and 08(32%) for first trimester, second trimester and third trimester respectively. Seroprevalence T. Gondii IgM and IgG were seen statistically insignificant (P>0.05) association to gestation age (1^st, 2^nd and 3^rd trimesters). IgG and IgM were statistically insignificant (P>0.05) in relation to age group of pregnant women. Seroprevalence of Toxoplasma IgG+ IgM– in pregnant women were 03(15.8%), 15(25.4%), and 08(32%) for first trimester, second trimester and third trimester respectively, these results indicate to past infection toxoplasmosis. Toxo IgG+ IgM+ was 02(3.4%), for second trimester, these results indicate to recent infection. Toxo IgG– IgM– was 16 (82.2%), 44(74.6%), and 17(68%) for first trimester, second trimester and third trimester respectively, these negative results indicate no prior exposure to Toxoplasma gondi iinfection, these individuals are presumed to be susceptible to a primary infection.