ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Slamet Isworo1 and Sulistyani2

1Environmental Health Department, Faculty of Health Science, Dian Nuswantoro University, Semarang, Indonesia.
2Environmental Health Department, Faculty of Public Health, Diponegoro University, Semarang, Indonesia.
J. Pure Appl. Microbiol., 2019, 13 (4): 1977-1984 | Article Number: 5873 | © The Author(s). 2019
Received: 03/09/2019 | Accepted: 28/09/2019 | Published: 04/12/2019

Increased antibiotic resistance spurs exploration of bioactive compounds as new antibiotic alternatives. Bacillus sp. is a symbiont bacterium which is a marine microorganism that has the potential to produce new bioactive compounds that can be developed as new antibiotics. This research is an experimental study aimed at identifying bioactive compounds by thin layer chromatography methods and testing the activity of bioactive compounds by probit analysis EPA probit analysis program version 1.5. in Artemia salina Leach. Bioactive compounds identified were compounded from the alkaloid group with the category of highly toxic several-irritating base on the EPA toxicity category and the highly hazardous base on The WHO toxicity category based on the environmental protection agency probit analysis program used for calculating LC / EC value of LC 50 = 169,520 g / l. Symbiont Bacillus sp. produce secondary metabolites in the form of bioactive which have the ability as anti-bacteria. As a new antibiotic alternative to overcome resistance, especially in methicillin-resistant bacteria.


Bacillus sp., Artemia salina Leach, Secondary metabolites, Bioactive, Highly toxic several – irritating, Symbiont, Highly hazardous, Brine shrimp lethality toxicity.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.