ISSN: 0973-7510

E-ISSN: 2581-690X

B. Ramanujam , G. Roopa, P. Karmakar and H. Basha
1National Bureau of Agriculturally Important Insects, Bangalore – 560 024, Karnataka, India.
J Pure Appl Microbiol. 2014;8(1):715-720
© The Author(s). 2014
Received: 10/04/2013 | Accepted: 03/06/2013 | Published: 28/02/2014

The present study describes the in vitro toxicity of extracellular partially purified soluble proteins (PPSPs) obtained from indigenous isolates of Beauveria bassiana and Metarhizium anisopliae on Spodoptera litura. Toxicity was determined by injecting PPSPs at six different concentrations into 5th instar larvae of S. litura. The larvae were susceptible to different protein concentrations in a dose-dependent manner. Among 77 isolates of B. bassiana, NBAII Bb-12, 47, 48 and 49 showed significantly higher mortality (80-87%) at the dose of 1.25 mg protein ml-1 with LC50 values of 0.35-0.85 mg ml-1. Among 55 isolates of M. anisopliae, NBAII Ma-4 and 42 showed similar mortality rates (80-87%) at 1.75 mg protein ml-1 with LC50 values of 0.49-0.71mg ml-1. Microscopic observations revealed the presence of dark melanized spots on dorsal, ventral surface and inner side of tracheal wall in treated larvae indicating the toxic effects of PPSPs.


Spodoptera litura, Beauveria bassiana, Metarhizium anisopliae, Insecticidal proteins, Toxicity

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.