ISSN: 0973-7510

E-ISSN: 2581-690X

Turki A. AL-Shammari1, Ali H. Bahkali2, Abdallah M. Elgorban1, Maged T. El-Kahky3 and Basheer A. Al-Sum2
1Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
2Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
3Plant Pathology Department, College of Agriculture, Mansoura University, Mansoura 35516, Egypt.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):199-207
© The Author(s). 2013
Received: 11/07/2013 | Accepted: 10/09/2013 | Published: 30/11/2013

The anti-nematode activity of Trichoderma longibrachiatum and Mortierella alpina was studied against Meloidogyne javanica. Treatment of eggs with T. longibrachiatum and M. alpina infected 89.3 and 90.3% eggs, respectively at 107cfu/ml. The both fungi decreased hatching rate of eggs by 8.9% (T. longibrachiatum) and 5.7% (M. alpina) and. Otherwise, the culture filtrates of T. longibrachiatum and M. alpina caused mortality of M. javanica second stage juveniles (J2) with 64.5 and 54.3% after 72 h of exposures, respectively. Under greenhouse conditions, all treatments reduced the disease severity and enhanced plant growth compared to untreated control. Both fungi reduced galls number/plant, number of egg masses/plant and the number of eggs/eggmass. Plant height, root length, fresh and dry weight/plant were significantly reduced because of infection with M. javanica, however the application of biocontrol agents recovered this reduction. Furthermore, they enhanced the growth parameters compared with the control. Our results proved that the application of different biocontrol agents not only has a toxic effect on M. javanica, but also enhances the plant growth, supplying many nutritional elements and induction the systemic resistance in plants. We demonstrated that application at a concentration of 106 or 107cfu/ml to soil is needed for sufficient biocontrol of M. javanica by T. longibrachiatum and M. alpina.


Meloidogyne javanica, T. longibrachiatum, Tomato

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© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.